{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["NCRR NIH HHS","Office of Extramural Research, National Institutes of Health","NIGMS NIH HHS"],"pagination":["1038"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8229658"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(6)"],"pubmed_abstract":["The NS1 protein of influenza A virus (IAV) plays important roles in viral pathogenesis and host immune response. Through a proteomic approach, we have identified RuvB-like proteins 1 and 2 (RuvBL1 and RuvBL2) as interacting partners of the NS1 protein of IAVs. Infection of human lung A549 cells with A/PR/8/34 (PR8) virus resulted in reductions in the protein levels of RuvBL2 but not RuvBL1. Further studies with RuvBL2 demonstrated that the NS1-RuvBL2 interaction is RNA-independent, and RuvBL2 binds the RNA-binding domain of the NS1. Infection of interferon (IFN)-deficient Vero cells with wild-type or delNS1 PR8 virus reduced RuvBL2 protein levels and induced apoptosis; delNS1 virus caused more reductions in RuvBL2 protein levels and induced more apoptosis than did wild-type virus. Knockdow"],"journal":["Viruses"],"pubmed_title":["RuvB-Like Protein 2 Interacts with the NS1 Protein of Influenza A Virus and Affects Apoptosis That Is Counterbalanced by Type I Interferons."],"pmcid":["PMC8229658"],"funding_grant_id":["R24 GM137786","P30 GM103450, 3P20RR015569-10S2, R24GM137786, P20GM121293, P20GM103429","P20 RR015569","P30 GM103450","P20 GM121293"],"pubmed_authors":["Du Y","Wang Y","Mackintosh SG","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"RuvB-Like Protein 2 Interacts with the NS1 Protein of Influenza A Virus and Affects Apoptosis That Is Counterbalanced by Type I Interferons.","description":"The NS1 protein of influenza A virus (IAV) plays important roles in viral pathogenesis and host immune response. Through a proteomic approach, we have identified RuvB-like proteins 1 and 2 (RuvBL1 and RuvBL2) as interacting partners of the NS1 protein of IAVs. Infection of human lung A549 cells with A/PR/8/34 (PR8) virus resulted in reductions in the protein levels of RuvBL2 but not RuvBL1. Further studies with RuvBL2 demonstrated that the NS1-RuvBL2 interaction is RNA-independent, and RuvBL2 binds the RNA-binding domain of the NS1. Infection of interferon (IFN)-deficient Vero cells with wild-type or delNS1 PR8 virus reduced RuvBL2 protein levels and induced apoptosis; delNS1 virus caused more reductions in RuvBL2 protein levels and induced more apoptosis than did wild-type virus. Knockdow","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-01T03:07:40.925Z","creation":"2022-02-10T19:56:19.729Z"},"accession":"S-EPMC8229658","cross_references":{"pubmed":["34072766"],"doi":["10.3390/v13061038"]}}