biostudies-literatureOrhan ESIRIC Montpellier Cancer2930https://www.ebi.ac.uk/biostudies/studies/S-EPMC8230762biostudies-literatureUnknown13(12)The RAD51 recombinase is a critical effector of Homologous Recombination (HR), which is an essential DNA repair mechanism for double-strand breaks. The RAD51 protein is recruited onto the DNA break by BRCA2 and forms homopolymeric filaments that invade the homologous chromatid and use it as a template for repair. RAD51 filaments are detectable by immunofluorescence as distinct foci in the cell nucleus, and their presence is a read out of HR proficiency. RAD51 is an essential gene, protecting cells from genetic instability. Its expression is low and tightly regulated in normal cells and, contrastingly, elevated in a large fraction of cancers, where its level of expression and activity have been linked with sensitivity to genotoxic treatment. In particular, BRCA-deficient tumors show reducedCancersRegulation of RAD51 at the Transcriptional and Functional Levels: What Prospects for Cancer Therapy?PMC8230762Grant INCa-DGOS-Inserm_12553Theillet CSardet CVelazquez COrhan ETabet IfalseRegulation of RAD51 at the Transcriptional and Functional Levels: What Prospects for Cancer Therapy?The RAD51 recombinase is a critical effector of Homologous Recombination (HR), which is an essential DNA repair mechanism for double-strand breaks. The RAD51 protein is recruited onto the DNA break by BRCA2 and forms homopolymeric filaments that invade the homologous chromatid and use it as a template for repair. RAD51 filaments are detectable by immunofluorescence as distinct foci in the cell nucleus, and their presence is a read out of HR proficiency. RAD51 is an essential gene, protecting cells from genetic instability. Its expression is low and tightly regulated in normal cells and, contrastingly, elevated in a large fraction of cancers, where its level of expression and activity have been linked with sensitivity to genotoxic treatment. In particular, BRCA-deficient tumors show reduced2021-01-01T00:00:00Z2021 Jun2026-04-08T09:18:08.878Z2022-02-10T18:04:36.816ZS-EPMC82307623420819510.3390/cancers13122930