biostudies-literature00540Hoffmann JDeutsche ForschungsgemeinschaftEuropean Research Council3964https://www.ebi.ac.uk/biostudies/studies/S-EPMC8233308biostudies-literatureUnknown12(1)The regulation of bone vasculature by chronic diseases, such as heart failure is unknown. Here, we describe the effects of myocardial infarction and post-infarction heart failure on the bone vascular cell composition. We demonstrate an age-independent loss of type H endothelium in heart failure after myocardial infarction in both mice and humans. Using single-cell RNA sequencing, we delineate the transcriptional heterogeneity of human bone marrow endothelium, showing increased expression of inflammatory genes, including IL1B and MYC, in ischemic heart failure. Endothelial-specific overexpression of MYC was sufficient to induce type H bone endothelial cells, whereas inhibition of NLRP3-dependent IL-1β production partially prevented the post-myocardial infarction loss of type H vasculature in mice. These results provide a rationale for using anti-inflammatory therapies to prevent or reverse the deterioration of bone vascular function in ischemic heart disease.Nature communicationsPost-myocardial infarction heart failure dysregulates the bone vascular niche.PMC8233308SFB 834 - B1773047SFB 834 - B6Abplanalp WTAssmus BMuhly-Reinholz MFischer AHoffmann JGlaser SFRasper TPotente MDimmeler SLuxan GZeiher AMJohn D54falsePost-myocardial infarction heart failure dysregulates the bone vascular niche.The regulation of bone vasculature by chronic diseases, such as heart failure is unknown. Here, we describe the effects of myocardial infarction and post-infarction heart failure on the bone vascular cell composition. We demonstrate an age-independent loss of type H endothelium in heart failure after myocardial infarction in both mice and humans. Using single-cell RNA sequencing, we delineate the transcriptional heterogeneity of human bone marrow endothelium, showing increased expression of inflammatory genes, including IL1B and MYC, in ischemic heart failure. Endothelial-specific overexpression of MYC was sufficient to induce type H bone endothelial cells, whereas inhibition of NLRP3-dependent IL-1β production partially prevented the post-myocardial infarction loss of type H vasculature in mice. These results provide a rationale for using anti-inflammatory therapies to prevent or reverse the deterioration of bone vascular function in ischemic heart disease.2021-01-01T00:00:00Z2021 Jun2024-10-17T20:51:50.594Z2022-02-10T18:33:24.904ZS-EPMC82333083417272010.1038/s41467-021-24045-4