{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Manstein F"],"funding":["European Union H2020 program to the project TECHNOBEAT","Deutsche Forschungsgemeinschaft","German Research Foundation; Cluster of Excellence REBIRTH","German Ministry for Education and Science"],"pagination":["1063-1080"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8235132"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(7)"],"pubmed_abstract":["To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in silico process modeling to overcome substantial, hPSC-specific hurdles toward their mass production. Perfused suspension culture (3D) of matrix-free hPSC aggregates in STBRs was applied to identify and control process-limiting parameters including pH, dissolved oxygen, glucose and lactate levels, and the obviation of osmolality peaks provoked by high density culture. Media supplements promoted single cell-based process inoculation and hydrodynamic aggregate size control. Wet lab-derived process characteristics enabled predictive in silico modeling as a new rational for hPSC cultivation. Consequently, hPSC line-independent maintenance "],"journal":["Stem cells translational medicine"],"pubmed_title":["High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling."],"pmcid":["PMC8235132"],"funding_grant_id":["01EK1602A","13N14086","ZW64/4-1","ZW64/4‐1","01EK1601A","668724","EXC 62/2"],"pubmed_authors":["Kalies S","Martin U","Zweigerdt R","Kropp C","Haase A","Olmer R","Franke A","Peitz M","Manstein F","Halloin C","Triebert W","Ullmann K","Sahabian A","Brustle O","Farr CM","Breitkreuz Y"],"additional_accession":[]},"is_claimable":false,"name":"High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling.","description":"To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in silico process modeling to overcome substantial, hPSC-specific hurdles toward their mass production. Perfused suspension culture (3D) of matrix-free hPSC aggregates in STBRs was applied to identify and control process-limiting parameters including pH, dissolved oxygen, glucose and lactate levels, and the obviation of osmolality peaks provoked by high density culture. Media supplements promoted single cell-based process inoculation and hydrodynamic aggregate size control. Wet lab-derived process characteristics enabled predictive in silico modeling as a new rational for hPSC cultivation. Consequently, hPSC line-independent maintenance ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-04T09:30:38.405Z","creation":"2022-02-10T16:49:32.473Z"},"accession":"S-EPMC8235132","cross_references":{"pubmed":["33660952"],"doi":["10.1002/sctm.20-0453"]}}