{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shi J"],"funding":["NICHD NIH HHS","European Research Council","NIDDK NIH HHS","Howard Hughes Medical Institute","NIEHS NIH HHS","NCI NIH HHS","NINDS NIH HHS","Wellcome Trust","NIGMS NIH HHS","Biotechnology and Biological Sciences Research Council"],"pagination":["424-436"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8236090"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(4)"],"pubmed_abstract":["Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA modifications that interfere with adapter ligation and reverse transcription processes, which prevent the detection of sncRNAs bearing these modifications. Here, we present PANDORA-seq (panoramic RNA display by overcoming RNA modification aborted sequencing), employing a combinatorial enzymatic treatment to remove key RNA modifications that block adapter ligation and reverse transcription. PANDORA-seq identified abundant modified sncRNAs-mostly transfer RNA-derived small RNAs (tsRNAs) and ribosomal RNA-derived small RNAs (rsRNAs)-that were pr"],"journal":["Nature cell biology"],"pubmed_title":["PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications."],"pmcid":["PMC8236090"],"funding_grant_id":["R01 NS113583","P50 HD098593","R01 ES032024","R01 GM124349","R35 GM128854","R01 HD092431","207415/Z/17/Z","P30 DK120515","P30 CA023100","P30 DK063491","669198"],"pubmed_authors":["Zhao W","Liu S","Zhang L","Zernicka-Goetz M","Cheloufi S","Zhao L","Guo J","Franklin R","Zhou C","Carrell DT","Mackinlay K","Qu Y","Chen X","Zhai Q","Wang Y","Yang XL","Yan M","Chen Q","Murn J","Gu W","Tan D","Zhou T","Cairns BR","Zhang Y","Shahbazi M","Schimmel P","Zhang X","Shi J","James ER","Kuhle B"],"additional_accession":[]},"is_claimable":false,"name":"PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications.","description":"Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA modifications that interfere with adapter ligation and reverse transcription processes, which prevent the detection of sncRNAs bearing these modifications. Here, we present PANDORA-seq (panoramic RNA display by overcoming RNA modification aborted sequencing), employing a combinatorial enzymatic treatment to remove key RNA modifications that block adapter ligation and reverse transcription. PANDORA-seq identified abundant modified sncRNAs-mostly transfer RNA-derived small RNAs (tsRNAs) and ribosomal RNA-derived small RNAs (rsRNAs)-that were pr","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-05-31T18:02:06.694Z","creation":"2025-02-18T23:37:18.305Z"},"accession":"S-EPMC8236090","cross_references":{"pubmed":["33820973"],"doi":["10.1038/s41556-021-00652-7"]}}