biostudies-literatureUnknown12Hoecker NDeutsche ForschungsgemeinschaftThe protein family 0016 (UPF0016) is conserved through evolution, and the few members characterized share a function in Mn²⁺ transport. So far, little is known about the history of these proteins in Eukaryotes. In <i>Arabidopsis thaliana</i> five such proteins, comprising four different subcellular localizations including chloroplasts, have been described, whereas non-photosynthetic Eukaryotes have only one. We used a phylogenetic approach to classify the eukaryotic proteins into two subgroups and performed gene-replacement studies to investigate UPF0016 genes of various origins. Replaceability can be scored readily in the Arabidopsis UPF0016 transporter mutant <i>pam71</i>, which exhibits a functional deficiency in photosystem II. The N-terminal region of the Arabidopsis PAM71 Frontiers in plant science697848https://www.ebi.ac.uk/biostudies/studies/S-EPMC8236900biostudies-literatureGene Replacement in Arabidopsis Reveals Manganese Transport as an Ancient Feature of Human, Plant and Cyanobacterial UPF0016 Proteins.PMC8236900Schmidt SBSchneider ALeister DMarino GHoecker NHennecke YSchrott SfalseGene Replacement in Arabidopsis Reveals Manganese Transport as an Ancient Feature of Human, Plant and Cyanobacterial UPF0016 Proteins.The protein family 0016 (UPF0016) is conserved through evolution, and the few members characterized share a function in Mn²⁺ transport. So far, little is known about the history of these proteins in Eukaryotes. In <i>Arabidopsis thaliana</i> five such proteins, comprising four different subcellular localizations including chloroplasts, have been described, whereas non-photosynthetic Eukaryotes have only one. We used a phylogenetic approach to classify the eukaryotic proteins into two subgroups and performed gene-replacement studies to investigate UPF0016 genes of various origins. Replaceability can be scored readily in the Arabidopsis UPF0016 transporter mutant <i>pam71</i>, which exhibits a functional deficiency in photosystem II. The N-terminal region of the Arabidopsis PAM71 2021-01-01T00:00:00Z20212026-05-09T07:25:16.945Z2022-02-10T17:35:50.624ZS-EPMC82369003419446210.3389/fpls.2021.697848