biostudies-literatureUnknown7(6)Umezu TSmoking Research FoundationThe present study used a binding assay to identify novel target biomolecules of <i>l</i>-menthol ([-]-menthol) that promote mouse ambulation. Among 88 different ligands to specific biomolecules examined, 0.1 mM <i>l</i>-menthol inhibited the binding of 13 ligands with relatively high inhibition rates. The assays showed that <i>l</i>-menthol acts on calcium channels, sodium channels, γ-aminobutyric acid type A (GABA_A) receptor, GABA transporter, dopamine transporter, dopamine D4 receptor, adenosine A2a receptor, α2A-adrenergic receptor, histamine H2 receptor, bombesin receptor, angiotensin AT1 receptor, vasopressin V2 receptor, and leukotriene B4 receptor over a similar concentration range. The inhibition constant (K_i) for <i>l</i>-menthol inhibition of binding of [<suHeliyone07329https://www.ebi.ac.uk/biostudies/studies/S-EPMC8237303biostudies-literatureIdentification of novel target molecules of <i>l</i>-menthol.PMC8237303Umezu TfalseIdentification of novel target molecules of <i>l</i>-menthol.The present study used a binding assay to identify novel target biomolecules of <i>l</i>-menthol ([-]-menthol) that promote mouse ambulation. Among 88 different ligands to specific biomolecules examined, 0.1 mM <i>l</i>-menthol inhibited the binding of 13 ligands with relatively high inhibition rates. The assays showed that <i>l</i>-menthol acts on calcium channels, sodium channels, γ-aminobutyric acid type A (GABA_A) receptor, GABA transporter, dopamine transporter, dopamine D4 receptor, adenosine A2a receptor, α2A-adrenergic receptor, histamine H2 receptor, bombesin receptor, angiotensin AT1 receptor, vasopressin V2 receptor, and leukotriene B4 receptor over a similar concentration range. The inhibition constant (K_i) for <i>l</i>-menthol inhibition of binding of [<su2021-01-01T00:00:00Z2021 Jun2026-05-09T07:59:18.975Z2022-02-10T17:07:48.196ZS-EPMC82373033419543210.1016/j.heliyon.2021.e07329