{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Talbot LJ"],"funding":["Rally Foundation","Assisi Foundation of Memphis","St. Jude Children's Research Hospital","NCI NIH HHS"],"pagination":["691741"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8239305"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["The outcome for metastatic pediatric osteosarcoma (OS) remains poor. Thus, there is an urgent need to develop novel therapies, and immunotherapy with CAR T cells has the potential to meet this challenge. However, there is a lack of preclinical models that mimic salient features of human disease including reliable development of metastatic disease post orthotopic OS cell injection. To overcome this roadblock, and also enable real-time imaging of metastatic disease, we took advantage of LM7 OS cells expressing firefly luciferase (LM7.ffLuc). LM7.ffLuc were implanted in a collagen mesh into the tibia of mice, and mice reliably developed orthotopic tumors and lung metastases as judged by bioluminescence imaging and histopathological analysis. Intratibial implantation also enabled surgical remo"],"journal":["Frontiers in immunology"],"pubmed_title":["A Novel Orthotopic Implantation Technique for Osteosarcoma Produces Spontaneous Metastases and Illustrates Dose-Dependent Efficacy of B7-H3-CAR T Cells."],"pmcid":["PMC8239305"],"funding_grant_id":["R25 CA023944","182154010","94-000R19","P01 CA096832","R50 CA211481"],"pubmed_authors":["Ross A","DeRenzo C","Tillman H","Talbot LJ","Davidoff A","Chabot A","Funk A","Gottschalk S","Nguyen P","Wagner J"],"additional_accession":[]},"is_claimable":false,"name":"A Novel Orthotopic Implantation Technique for Osteosarcoma Produces Spontaneous Metastases and Illustrates Dose-Dependent Efficacy of B7-H3-CAR T Cells.","description":"The outcome for metastatic pediatric osteosarcoma (OS) remains poor. Thus, there is an urgent need to develop novel therapies, and immunotherapy with CAR T cells has the potential to meet this challenge. However, there is a lack of preclinical models that mimic salient features of human disease including reliable development of metastatic disease post orthotopic OS cell injection. To overcome this roadblock, and also enable real-time imaging of metastatic disease, we took advantage of LM7 OS cells expressing firefly luciferase (LM7.ffLuc). LM7.ffLuc were implanted in a collagen mesh into the tibia of mice, and mice reliably developed orthotopic tumors and lung metastases as judged by bioluminescence imaging and histopathological analysis. Intratibial implantation also enabled surgical remo","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-04T09:11:05.15Z","creation":"2022-02-10T17:59:01.194Z"},"accession":"S-EPMC8239305","cross_references":{"pubmed":["34211478"],"doi":["10.3389/fimmu.2021.691741"]}}