biostudies-literatureYuan ZNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesGeorgia Research AllianceGeorgia State University5317-5326https://www.ebi.ac.uk/biostudies/studies/S-EPMC8248650biostudies-literatureUnknown93(12)Ruthenium-based CO-releasing molecules (CO-RMs), CORM-2 and CORM-3, have been widely used as surrogates of CO for studying its biological effects <i>in vitro</i> and <i>in vivo</i> with much success. However, several previous solution-phase and <i>in vitro</i> studies have revealed the ability of such CO-RMs to chemically modify proteins and reduce aromatic nitro groups due to their intrinsic chemical reactivity under certain conditions. In our own work of studying the cytoprotective effects of CO donors, we were in need of assessing chemical factors that could impact the interpretation of results from CO donors including CORM-2,3 in various <i>in vitro</i> assays. For this, we examined the effects of CORM-2,3 toward representative reagents commonly used in various bioassays including resaAnalytical chemistryChemical Reactivities of Two Widely Used Ruthenium-Based CO-Releasing Molecules with a Range of Biologically Important Reagents and Molecules.PMC8248650R01DK119202R01 DK119202Yang XWang BTripathi RYe YYuan ZfalseChemical Reactivities of Two Widely Used Ruthenium-Based CO-Releasing Molecules with a Range of Biologically Important Reagents and Molecules.Ruthenium-based CO-releasing molecules (CO-RMs), CORM-2 and CORM-3, have been widely used as surrogates of CO for studying its biological effects <i>in vitro</i> and <i>in vivo</i> with much success. However, several previous solution-phase and <i>in vitro</i> studies have revealed the ability of such CO-RMs to chemically modify proteins and reduce aromatic nitro groups due to their intrinsic chemical reactivity under certain conditions. In our own work of studying the cytoprotective effects of CO donors, we were in need of assessing chemical factors that could impact the interpretation of results from CO donors including CORM-2,3 in various <i>in vitro</i> assays. For this, we examined the effects of CORM-2,3 toward representative reagents commonly used in various bioassays including resa2021-01-01T00:00:00Z2021 Mar2025-04-04T20:16:15.749Z2025-04-04T20:16:15.749ZS-EPMC82486503374526910.1021/acs.analchem.1c00533