{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20"],"submitter":["Cirksena K"],"funding":["Deutsche Forschungsgemeinschaft","Bundesministerium fur Bildung und Forschung Dienststelle Bonn"],"pubmed_abstract":["C-mannosylation is a modification of tryptophan residues with a single mannose and can affect protein folding, secretion, and/or function. To date, only a few proteins have been demonstrated to be C-mannosylated, and studies that globally assess protein C-mannosylation are scarce. To interrogate the C-mannosylome of human induced pluripotent stem cells, we compared the secretomes of CRISPR-Cas9 mutants lacking either the C-mannosyltransferase DPY19L1 or DPY19L3 to WT human induced pluripotent stem cells using MS-based quantitative proteomics. The secretion of numerous proteins was reduced in these mutants, including that of A Disintegrin And Metalloproteinase with ThromboSpondin Motifs 16 (ADAMTS16), an extracellular protease that was previously reported to be essential for optic fissure f"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["100092"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8256286"],"repository":["biostudies-literature"],"pubmed_title":["The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development."],"pmcid":["PMC8256286"],"pubmed_authors":["Cirksena K","Bakker H","Buettner FFR","Friedrich A","Ruppert T","Todt D","Kuss AW","Jensen LR","Thumberger T","Hutte HJ","Sakson R","Weiss S","Shcherbakova A","Wittbrodt J"],"additional_accession":[]},"is_claimable":false,"name":"The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development.","description":"C-mannosylation is a modification of tryptophan residues with a single mannose and can affect protein folding, secretion, and/or function. To date, only a few proteins have been demonstrated to be C-mannosylated, and studies that globally assess protein C-mannosylation are scarce. To interrogate the C-mannosylome of human induced pluripotent stem cells, we compared the secretomes of CRISPR-Cas9 mutants lacking either the C-mannosyltransferase DPY19L1 or DPY19L3 to WT human induced pluripotent stem cells using MS-based quantitative proteomics. The secretion of numerous proteins was reduced in these mutants, including that of A Disintegrin And Metalloproteinase with ThromboSpondin Motifs 16 (ADAMTS16), an extracellular protease that was previously reported to be essential for optic fissure f","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-13T17:01:15.403Z","creation":"2026-05-01T03:05:46.734Z"},"accession":"S-EPMC8256286","cross_references":{"pubmed":["33975020"],"doi":["10.1016/j.mcpro.2021.100092"]}}