{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Alcock TD"],"funding":["Slovenian Research Agency","Renewable Industrial Products from Rapeseed","Rural and Environment Science and Analytical Services Division","Crop Improvement Research Club","Delivering Low-Cost, High-Throughput Root Phenotyping Screens for Arable Crops","Biotechnology and Biological Sciences Research Council","Scottish Government"],"pagination":["1616-1631"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8260142"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["186(3)"],"pubmed_abstract":["Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thalia"],"journal":["Plant physiology"],"pubmed_title":["Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa."],"pmcid":["PMC8260142"],"funding_grant_id":["N1-0105","BB/N023927/1","J7-9398","P1-0112","BB/L002124/1","P1-0212","BB/L027739/2","N7-0077","N1-0090","BB/G014159/1","BB/G014159/2","BBSSE200613215","BB-G013969-1","BB/J019631/1","J7-9418","BB/N004302/1"],"pubmed_authors":["Reyt G","Vogel-Mikus K","Hayden R","Irwin JA","Pongrac P","Alcock TD","Ostergaard L","Thomas CL","Wilson L","Stephenson P","Wilson M","Broadley MR","King GJ","Moore C","Hammond JP","Salt DE","Graham NS","O Lochlainn S","Kelemen M","White PJ"],"additional_accession":[]},"is_claimable":false,"name":"Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa.","description":"Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thalia","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2026-04-30T03:12:54.97Z","creation":"2022-02-11T13:04:46.507Z"},"accession":"S-EPMC8260142","cross_references":{"pubmed":["33831190"],"doi":["10.1093/plphys/kiab150"]}}