biostudies-literatureHanson PMedical Research Council145470https://www.ebi.ac.uk/biostudies/studies/S-EPMC8262470biostudies-literatureUnknown6(12)<h4>Background</h4>Dietary sodium intake mismatches urinary sodium excretion over prolonged periods. Our aims were to localize and quantify electrostatically bound sodium within human skin using triple-quantum-filtered (TQF) protocols for MRI and magnetic resonance spectroscopy (MRS) and to explore dermal sodium in type 2 diabetes mellitus (T2D).<h4>Methods</h4>We recruited adult participants with T2D (n = 9) and euglycemic participants with no history of diabetes mellitus (n = 8). All had undergone lower limb amputations or abdominal skin reduction surgery for clinical purposes. We used 20 μm in-plane resolution 1H MRI to visualize anatomical skin regions ex vivo from skin biopsies taken intraoperatively, 23Na TQF MRI/MRS to explore distribution and quantification of freely dissolved and JCI insightSodium in the dermis colocates to glycosaminoglycan scaffold, with diminishment in type 2 diabetes mellitus.PMC8262470MC_PC_15074Hanson PJames SO'Hare JPMeersmann TBarber TMPavlovskaya GERandeva HSPhilp CJfalseSodium in the dermis colocates to glycosaminoglycan scaffold, with diminishment in type 2 diabetes mellitus.<h4>Background</h4>Dietary sodium intake mismatches urinary sodium excretion over prolonged periods. Our aims were to localize and quantify electrostatically bound sodium within human skin using triple-quantum-filtered (TQF) protocols for MRI and magnetic resonance spectroscopy (MRS) and to explore dermal sodium in type 2 diabetes mellitus (T2D).<h4>Methods</h4>We recruited adult participants with T2D (n = 9) and euglycemic participants with no history of diabetes mellitus (n = 8). All had undergone lower limb amputations or abdominal skin reduction surgery for clinical purposes. We used 20 μm in-plane resolution 1H MRI to visualize anatomical skin regions ex vivo from skin biopsies taken intraoperatively, 23Na TQF MRI/MRS to explore distribution and quantification of freely dissolved and 2021-01-01T00:00:00Z2021 Jun2026-07-16T11:10:17.484Z2022-02-10T19:33:21.559ZS-EPMC82624703400380110.1172/jci.insight.145470