biostudies-literatureLiu YNHLBI NIH HHSNational Institutes of Health14078https://www.ebi.ac.uk/biostudies/studies/S-EPMC8263753biostudies-literatureUnknown11(1)Murine animal models are an established standard in translational research and provides a potential platform for studying heart valve disease. To date, studies on heart valve disease using murine models have been hindered by a lack of appropriate methodologies due to their small scale. In the present study, we developed a multi-scale, imaging-based approach to extract the functional structure and geometry for the murine heart valve. We chose the pulmonary valve (PV) to study, due to its importance in congenital heart valve disease. Excised pulmonary outflow tracts from eleven 1-year old C57BL/6J mice were fixed at 10, 20, and 30 mmHg to simulate physiological loading. Micro-computed tomography was used to reconstruct the 3D organ-level PV geometry, which was then spatially correlated with Scientific reportsOn the shape and structure of the murine pulmonary heart valve.PMC8263753HL142504R01 HL142504Liu HLiu YBreuer CKFeng XMcComb DWSacks MSfalseOn the shape and structure of the murine pulmonary heart valve.Murine animal models are an established standard in translational research and provides a potential platform for studying heart valve disease. To date, studies on heart valve disease using murine models have been hindered by a lack of appropriate methodologies due to their small scale. In the present study, we developed a multi-scale, imaging-based approach to extract the functional structure and geometry for the murine heart valve. We chose the pulmonary valve (PV) to study, due to its importance in congenital heart valve disease. Excised pulmonary outflow tracts from eleven 1-year old C57BL/6J mice were fixed at 10, 20, and 30 mmHg to simulate physiological loading. Micro-computed tomography was used to reconstruct the 3D organ-level PV geometry, which was then spatially correlated with 2021-01-01T00:00:00Z2021 Jul2025-04-19T02:11:04.158Z2022-02-10T18:34:33.233ZS-EPMC82637533423423110.1038/s41598-021-93513-0