biostudies-literatureSun SSocial Development and Scientific-technological Foundation of Zhenjiang1209-1219https://www.ebi.ac.uk/biostudies/studies/S-EPMC8265791biostudies-literatureUnknown20(12)Ankylosing spondylitis (AS) is a chronic autoimmune disease in which let-7i has been studied to involved. But, whether let-7i-3p could regulate osteoblast differentiation in AS remains unclear. This research targeted to decipher the impact of let-7i-3p on AS progression by modulating pyruvate dehydrogenase kinase 1 (PDK1). The bone mineral density of femur and lumbar vertebra and the maximum loading and bending elastic modulus of tibia, tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP)-3, osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in serum of AS mice, the pathological condition of synovial tissue were determined via let-7i-3p inhibitor and OE-PDK1 in animal experiment. Also, the cell viability and ALP activity were measured by let-7i-3p iCell cycle (Georgetown, Tex.)MicroRNA let-7i-3p affects osteoblast differentiation in ankylosing spondylitis via targeting PDK1.PMC8265791SH2018040Liu HZhu ZSun SKong DXu YZhou ZWang LfalseMicroRNA let-7i-3p affects osteoblast differentiation in ankylosing spondylitis via targeting PDK1.Ankylosing spondylitis (AS) is a chronic autoimmune disease in which let-7i has been studied to involved. But, whether let-7i-3p could regulate osteoblast differentiation in AS remains unclear. This research targeted to decipher the impact of let-7i-3p on AS progression by modulating pyruvate dehydrogenase kinase 1 (PDK1). The bone mineral density of femur and lumbar vertebra and the maximum loading and bending elastic modulus of tibia, tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP)-3, osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in serum of AS mice, the pathological condition of synovial tissue were determined via let-7i-3p inhibitor and OE-PDK1 in animal experiment. Also, the cell viability and ALP activity were measured by let-7i-3p i2021-01-01T00:00:00Z2021 Jun2025-04-19T20:44:43.041Z2025-04-19T20:44:43.041ZS-EPMC82657913404831110.1080/15384101.2021.1930680