{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shi X"],"funding":["American Heart Association","NIDCR NIH HHS","American Heart Association-American Stroke Association","University of California, San Francisco","National Institutes of Health","Chan Zuckerberg","Damon Runyon Cancer Research Foundation","David and Lucile Packard Foundation","NIH HHS","NIGMS NIH HHS","National Science Foundation"],"pagination":["e202105067"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8266563"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["220(9)"],"pubmed_abstract":["Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ."],"journal":["The Journal of cell biology"],"pubmed_title":["Label-retention expansion microscopy."],"pmcid":["PMC8266563"],"funding_grant_id":["R01GM124334","K99 GM126136","DP2 OD008479","DP2OD008479","19PRE3480616","R00 GM126136","K99GM126136/R00GM126136","R01 GM124334","1650113","DRG2168-13","R01 DE029454"],"pubmed_authors":["Chen J","Tran AA","Huang EJ","Li Q","Lin Z","Dai Z","Shi X","McColloch AR","Kumar D","Seiple IB","Ramirez AD","Reiter JF","Wang X","Feng S","Huang B","Chow TT"],"additional_accession":[]},"is_claimable":false,"name":"Label-retention expansion microscopy.","description":"Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-19T20:43:18.028Z","creation":"2025-04-19T20:43:18.028Z"},"accession":"S-EPMC8266563","cross_references":{"pubmed":["34228783"],"doi":["10.1083/jcb.202105067"]}}