{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Martinez J"],"pubmed_abstract":["The aim of this work was to study the value of the main allergen Asp n 3 of <i>Aspergillus niger</i> as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of <i>A. niger</i> as a contaminant and cause of spoilage of <i>Mangifera indica.</i> Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of <i>A. niger</i> was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region "],"journal":["Frontiers in microbiology"],"pagination":["663323"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8273346"],"repository":["biostudies-literature"],"pubmed_title":["The <i>Aspergillus niger</i> Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in <i>Mangifera indica</i> Fruit."],"pmcid":["PMC8273346"],"pubmed_authors":["Martinez J","Sanchez P","Sunen E","Velez-Del-Burgo A","Postigo I","Nevado A","Gabriel M"],"additional_accession":[]},"is_claimable":false,"name":"The <i>Aspergillus niger</i> Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in <i>Mangifera indica</i> Fruit.","description":"The aim of this work was to study the value of the main allergen Asp n 3 of <i>Aspergillus niger</i> as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of <i>A. niger</i> as a contaminant and cause of spoilage of <i>Mangifera indica.</i> Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of <i>A. niger</i> was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-05-31T23:47:03.64Z","creation":"2025-05-31T23:47:03.64Z"},"accession":"S-EPMC8273346","cross_references":{"pubmed":["34262539"],"doi":["10.3389/fmicb.2021.663323"]}}