biostudies-literatureUnknown12Martinez JThe aim of this work was to study the value of the main allergen Asp n 3 of <i>Aspergillus niger</i> as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of <i>A. niger</i> as a contaminant and cause of spoilage of <i>Mangifera indica.</i> Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of <i>A. niger</i> was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region Frontiers in microbiology663323https://www.ebi.ac.uk/biostudies/studies/S-EPMC8273346biostudies-literatureThe <i>Aspergillus niger</i> Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in <i>Mangifera indica</i> Fruit.PMC8273346Martinez JSanchez PSunen EVelez-Del-Burgo APostigo INevado AGabriel MfalseThe <i>Aspergillus niger</i> Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in <i>Mangifera indica</i> Fruit.The aim of this work was to study the value of the main allergen Asp n 3 of <i>Aspergillus niger</i> as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of <i>A. niger</i> as a contaminant and cause of spoilage of <i>Mangifera indica.</i> Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of <i>A. niger</i> was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region 2021-01-01T00:00:00Z20212025-05-31T23:47:03.64Z2025-05-31T23:47:03.64ZS-EPMC82733463426253910.3389/fmicb.2021.663323