biostudies-literatureUnknown9Bessonnet TDespite the success of some nitrilases in industrial applications, there is a constant demand to broaden the catalog of these hydrolases, especially robust ones with high operational stability. By using the criteria of thermoresistance to screen a collection of candidate enzymes heterologously expressed in <i>Escherichia coli</i>, the enzyme Nit <i>_<i>phym</i> </i> from the mesophilic organism <i>Paraburkholderia phymatum</i> was selected and further characterized. Its quick and efficient purification by heat treatment is of major interest for large-scale applications. The purified nitrilase displayed a high thermostability with 90% of remaining activity after 2 days at 30°C and a half-life of 18 h at 60°C, together with a broad pH range of 5.5-8.5. Its high resistance to variouFrontiers in bioengineering and biotechnology686362https://www.ebi.ac.uk/biostudies/studies/S-EPMC8280356biostudies-literaturePurification and Characterization of Nit <i>_<i>phym</i> </i> , a Robust Thermostable Nitrilase From <i>Paraburkholderia phymatum</i>.PMC8280356Vergne-Vaxelaire CBessonnet TMariage APellouin Vde Berardinis VPetit JLZaparucha ADebard AfalsePurification and Characterization of Nit <i>_<i>phym</i> </i> , a Robust Thermostable Nitrilase From <i>Paraburkholderia phymatum</i>.Despite the success of some nitrilases in industrial applications, there is a constant demand to broaden the catalog of these hydrolases, especially robust ones with high operational stability. By using the criteria of thermoresistance to screen a collection of candidate enzymes heterologously expressed in <i>Escherichia coli</i>, the enzyme Nit <i>_<i>phym</i> </i> from the mesophilic organism <i>Paraburkholderia phymatum</i> was selected and further characterized. Its quick and efficient purification by heat treatment is of major interest for large-scale applications. The purified nitrilase displayed a high thermostability with 90% of remaining activity after 2 days at 30°C and a half-life of 18 h at 60°C, together with a broad pH range of 5.5-8.5. Its high resistance to variou2021-01-01T00:00:00Z20212026-05-08T07:57:18.28Z2022-02-10T21:07:54.637ZS-EPMC82803563427758610.3389/fbioe.2021.686362