biostudies-literatureUnknownMuecksch FNIAID NIH HHS<h4>Background</h4>Serological assays are being deployed to monitor antibody responses in SARS-CoV-2 convalescents and vaccine recipients. There is a need to determine whether such assays can predict immunity, as antibody levels wane and viral variants emerge.<h4>Methods</h4>We measured antibodies in a cohort of SARS-CoV-2 infected patients using several high-throughput serological tests and functional neutralization assays. The effects of time and spike protein sequence variation on the performance and predictive value of the various assays was assessed.<h4>Findings</h4>Neutralizing antibody titers decreased over the first few months post-infection but stabilized thereafter, at about 30% of the level observed shortly after infection. Serological assays commonly used to measure antibodies medRxiv : the preprint server for health sciences2021.07.02.21259939https://www.ebi.ac.uk/biostudies/studies/S-EPMC8282113biostudies-literatureLongitudinal variation in SARS-CoV-2 antibody levels and emergence of viral variants: implications for the ability of serological assays to predict immunity.PMC8282113R01 AI078788R01 AI050111Malloy KZhang FBieniasz PHatziioannou TSchmidt FJenks SMcCance KSquires MMavin STempleton KMuecksch FBatchelor BRichardson CBurns AFurrie EGodber IJarvis LMacGuire JWise HfalseLongitudinal variation in SARS-CoV-2 antibody levels and emergence of viral variants: implications for the ability of serological assays to predict immunity.<h4>Background</h4>Serological assays are being deployed to monitor antibody responses in SARS-CoV-2 convalescents and vaccine recipients. There is a need to determine whether such assays can predict immunity, as antibody levels wane and viral variants emerge.<h4>Methods</h4>We measured antibodies in a cohort of SARS-CoV-2 infected patients using several high-throughput serological tests and functional neutralization assays. The effects of time and spike protein sequence variation on the performance and predictive value of the various assays was assessed.<h4>Findings</h4>Neutralizing antibody titers decreased over the first few months post-infection but stabilized thereafter, at about 30% of the level observed shortly after infection. Serological assays commonly used to measure antibodies 2021-01-01T00:00:00Z2021 Jul2026-06-19T03:18:12.936Z2022-02-11T16:05:15.757ZS-EPMC82821133426852410.1101/2021.07.02.21259939