{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Grigoraki L"],"funding":["Medical Research Council","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["e1009556"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8284791"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(7)"],"pubmed_abstract":["Insecticide resistance in Anopheles mosquitoes is a major obstacle in maintaining the momentum in reducing the malaria burden; mitigating strategies require improved understanding of the underlying mechanisms. Mutations in the target site of insecticides (the voltage gated sodium channel for the most widely used pyrethroid class) and over-expression of detoxification enzymes are commonly reported, but their relative contribution to phenotypic resistance remain poorly understood. Here we present a genome editing pipeline to introduce single nucleotide polymorphisms in An. gambiae which we have used to study the effect of the classical kdr mutation L1014F (L995F based on An. gambiae numbering), one of the most widely distributed resistance alleles. Introduction of 1014F in an otherwise fully"],"journal":["PLoS genetics"],"pubmed_title":["CRISPR/Cas9 modified An. gambiae carrying kdr mutation L1014F functionally validate its contribution in insecticide resistance and combined effect with metabolic enzymes."],"pmcid":["PMC8284791"],"funding_grant_id":["215894/Z/19/Z","BB/F021933/1","MR/V001264/1"],"pubmed_authors":["Cowlishaw R","Ranson H","Nolan T","Lycett G","Donnelly M","Grigoraki L"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR/Cas9 modified An. gambiae carrying kdr mutation L1014F functionally validate its contribution in insecticide resistance and combined effect with metabolic enzymes.","description":"Insecticide resistance in Anopheles mosquitoes is a major obstacle in maintaining the momentum in reducing the malaria burden; mitigating strategies require improved understanding of the underlying mechanisms. Mutations in the target site of insecticides (the voltage gated sodium channel for the most widely used pyrethroid class) and over-expression of detoxification enzymes are commonly reported, but their relative contribution to phenotypic resistance remain poorly understood. Here we present a genome editing pipeline to introduce single nucleotide polymorphisms in An. gambiae which we have used to study the effect of the classical kdr mutation L1014F (L995F based on An. gambiae numbering), one of the most widely distributed resistance alleles. Introduction of 1014F in an otherwise fully","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-26T06:40:39.806Z","creation":"2022-02-10T23:57:19.063Z"},"accession":"S-EPMC8284791","cross_references":{"pubmed":["34228718"],"doi":["10.1371/journal.pgen.1009556"]}}