{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bai H"],"funding":["NIEHS NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2793-2801"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8285079"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["413(10)"],"pubmed_abstract":["Three-dimensional (3D) mass spectrometry imaging (MSI) has become a growing frontier as it has the potential to provide a 3D representation of analytes in a label-free, untargeted, and chemically specific manner. The most common 3D MSI is accomplished by the reconstruction of 2D MSI from serial cryosections; however, this presents significant challenges in image alignment and registration. An alternative method would be to sequentially image a sample by consecutive ablation events to create a 3D image. In this study, we describe the use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue. Depth resolution using different laser energy levels was explored with a confocal laser scanni"],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry."],"pmcid":["PMC8285079"],"funding_grant_id":["R01 GM087964","R01GM087964","P30 ES025128"],"pubmed_authors":["Muddiman DC","Linder KE","Bai H"],"additional_accession":[]},"is_claimable":false,"name":"Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry.","description":"Three-dimensional (3D) mass spectrometry imaging (MSI) has become a growing frontier as it has the potential to provide a 3D representation of analytes in a label-free, untargeted, and chemically specific manner. The most common 3D MSI is accomplished by the reconstruction of 2D MSI from serial cryosections; however, this presents significant challenges in image alignment and registration. An alternative method would be to sequentially image a sample by consecutive ablation events to create a 3D image. In this study, we describe the use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue. Depth resolution using different laser energy levels was explored with a confocal laser scanni","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-04T20:16:22.262Z","creation":"2025-04-04T20:16:22.262Z"},"accession":"S-EPMC8285079","cross_references":{"pubmed":["33388847"],"doi":["10.1007/s00216-020-03105-6"]}}