{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kong H"],"funding":["Youth Innovation Promotion Association, CAS","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["1218-1227"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8288996"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(7)"],"pubmed_abstract":["Spatial resolution defines the physical limit of microscopes for probing biomolecular localization and interactions in cells. Whereas synchrotron-based X-ray microscopy (XRM) represents a unique approach for imaging a whole cell with nanoscale resolution due to its intrinsic nanoscale resolution and great penetration ability, existing approaches to label biomolecules rely on the use of exogenous tags that are multi-step and error-prone. Here, we repurpose engineered peroxidases as genetically encoded X-ray-sensitive tags (GXET) for site-specific labeling of protein-of-interest in mammalian cells. We find that 3,3<sup>'</sup>-diaminobenzidine (DAB) polymers that are in-situ catalytically formed by fusion-expressed peroxidases are visible under XRM. Using this new tag, we imaged the protein "],"journal":["National science review"],"pubmed_title":["Genetically encoded X-ray cellular imaging for nanoscale protein localization."],"pmcid":["PMC8288996"],"funding_grant_id":["21390414","2012205","2016YFA0400902","2016236","11675251","21834007"],"pubmed_authors":["Xia K","Li J","Tai R","Yan Q","Zhang J","Hu J","Zhu Y","Wang J","Kong H","Fan C","Shin HJ","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Genetically encoded X-ray cellular imaging for nanoscale protein localization.","description":"Spatial resolution defines the physical limit of microscopes for probing biomolecular localization and interactions in cells. Whereas synchrotron-based X-ray microscopy (XRM) represents a unique approach for imaging a whole cell with nanoscale resolution due to its intrinsic nanoscale resolution and great penetration ability, existing approaches to label biomolecules rely on the use of exogenous tags that are multi-step and error-prone. Here, we repurpose engineered peroxidases as genetically encoded X-ray-sensitive tags (GXET) for site-specific labeling of protein-of-interest in mammalian cells. We find that 3,3<sup>'</sup>-diaminobenzidine (DAB) polymers that are in-situ catalytically formed by fusion-expressed peroxidases are visible under XRM. Using this new tag, we imaged the protein ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2025-04-19T21:17:46.528Z","creation":"2025-04-19T21:17:46.528Z"},"accession":"S-EPMC8288996","cross_references":{"pubmed":["34692146"],"doi":["10.1093/nsr/nwaa055"]}}