biostudies-literatureMangiola SNational Health and Medical Research Council846https://www.ebi.ac.uk/biostudies/studies/S-EPMC8296706biostudies-literatureUnknown21(1)<h4>Background</h4>Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities.<h4>Results</h4>In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence waBMC cancerTranscriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression.PMC82967061054618Modrak MKeam SPNasa CStuchbery RPapenfuss ATBlashki DNeeson PJPal BCostello AJLister NHovens CMMcCoy PCorcoran NMMonard SMangiola SKerger MDundee PHuntington NDChow KLe Page MPeters JWilliams SGSouza-Fonseca-Guimaraes FfalseTranscriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression.<h4>Background</h4>Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities.<h4>Results</h4>In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence wa2021-01-01T00:00:00Z2021 Jul2026-07-16T08:20:13.24Z2025-05-29T20:01:06.392ZS-EPMC82967063429407310.1186/s12885-021-08529-6