biostudies-literatureJontvedt Jorgensen MNorges ForskningsrådHelse Sør-Øst RHF669623https://www.ebi.ac.uk/biostudies/studies/S-EPMC8299478biostudies-literatureUnknown11<h4>Introduction</h4>Eicosanoids and intracellular signaling pathways are potential targets for host-directed therapy (HDT) in tuberculosis (TB). We have explored the effect of cyclooxygenase 2 inhibitor (COX-2i) treatment on eicosanoid levels and signaling pathways in monocytes.<h4>Methods</h4>Peripheral blood mononuclear cells isolated from TB patients included in a randomized phase I clinical trial of standard TB treatment with (n=21) or without (n=18) adjunctive COX-2i (etoricoxib) were analyzed at baseline, day 14 and day 56. Plasma eicosanoids were analyzed by ELISA and liquid chromatography-mass spectrometry (LC-MS), plasma cytokines by multiplex, and monocyte signaling by phospho-flow with a defined set of phospho-specific antibodies.<h4>Results</h4>Lipoxygenase (LOX)-derived produFrontiers in cellular and infection microbiologyPlasma LOX-Products and Monocyte Signaling Is Reduced by Adjunctive Cyclooxygenase-2 Inhibitor in a Phase I Clinical Trial of Tuberculosis Patients.PMC8299478GlobVac no 234493Layre EAass HCDMortensen RTonby KJenum STasken KDyrhol-Riise AMJontvedt Jorgensen MKvale DNore KGNigou JfalsePlasma LOX-Products and Monocyte Signaling Is Reduced by Adjunctive Cyclooxygenase-2 Inhibitor in a Phase I Clinical Trial of Tuberculosis Patients.<h4>Introduction</h4>Eicosanoids and intracellular signaling pathways are potential targets for host-directed therapy (HDT) in tuberculosis (TB). We have explored the effect of cyclooxygenase 2 inhibitor (COX-2i) treatment on eicosanoid levels and signaling pathways in monocytes.<h4>Methods</h4>Peripheral blood mononuclear cells isolated from TB patients included in a randomized phase I clinical trial of standard TB treatment with (n=21) or without (n=18) adjunctive COX-2i (etoricoxib) were analyzed at baseline, day 14 and day 56. Plasma eicosanoids were analyzed by ELISA and liquid chromatography-mass spectrometry (LC-MS), plasma cytokines by multiplex, and monocyte signaling by phospho-flow with a defined set of phospho-specific antibodies.<h4>Results</h4>Lipoxygenase (LOX)-derived produ2021-01-01T00:00:00Z20212026-05-08T18:49:37.048Z2022-02-10T23:41:51.099ZS-EPMC82994783430719410.3389/fcimb.2021.669623