biostudies-literatureRodrigues AMFundação para a Ciência e a TecnologiaConselho Nacional de Desenvolvimento Científico e TecnológicoHorizon 2020427https://www.ebi.ac.uk/biostudies/studies/S-EPMC8303404biostudies-literatureUnknown11(7)Climate change scenarios pose major threats to many crops worldwide, including coffee. We explored the primary metabolite responses in two Coffea genotypes, C. canephora cv. Conilon Clone 153 and C. arabica cv. Icatu, grown at normal (aCO2) or elevated (eCO2) CO2 concentrations of 380 or 700 ppm, respectively, under well-watered (WW), moderate (MWD), or severe (SWD) water deficit conditions, in order to assess coffee responses to drought and how eCO2 can influence such responses. Primary metabolites were analyzed with a gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). A total of 48 primary metabolites were identified in both genotypes (23 amino acids and derivatives, 10 organic acids, 11 sugars, and 4 other metabolites), with differences recorded in boMetabolitesPrimary Metabolite Profile Changes in Coffea spp. Promoted by Single and Combined Exposure to Drought and Elevated CO2 Concentration.PMC8303404PD/BD/114417/2016UIDB/00239/2020PD/00035/2013UID/Multi/04551/2013PTDC/ASP-AGR/31257/2017CRA-RED-00053-16727934IF/00376/2012/CP0165/CT0003UIDP/04035/2020PD/BD/113475/2015Rodrigues AMRamalho JCJorge TAntonio CMarques IRibeiro-Barros AIOsorio SLidon FCPott DMDaMatta FMfalsePrimary Metabolite Profile Changes in Coffea spp. Promoted by Single and Combined Exposure to Drought and Elevated CO2 Concentration.Climate change scenarios pose major threats to many crops worldwide, including coffee. We explored the primary metabolite responses in two Coffea genotypes, C. canephora cv. Conilon Clone 153 and C. arabica cv. Icatu, grown at normal (aCO2) or elevated (eCO2) CO2 concentrations of 380 or 700 ppm, respectively, under well-watered (WW), moderate (MWD), or severe (SWD) water deficit conditions, in order to assess coffee responses to drought and how eCO2 can influence such responses. Primary metabolites were analyzed with a gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). A total of 48 primary metabolites were identified in both genotypes (23 amino acids and derivatives, 10 organic acids, 11 sugars, and 4 other metabolites), with differences recorded in bo2021-01-01T00:00:00Z2021 Jun2025-04-04T23:27:07.908Z2022-02-10T23:02:00.707ZS-EPMC83034043420962410.3390/metabo11070427