{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van der Spek SJF"],"funding":["ZonMw"],"pagination":["1588"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8304546"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(7)"],"pubmed_abstract":["Alzheimer's disease (AD) is the most common neurodegenerative disorder in the human population, for which there is currently no cure. The cause of AD is unknown; however, the toxic effects of amyloid-β (Aβ) are believed to play a role in its onset. To investigate this, we examined changes in global protein levels in a hippocampal synaptosome fraction of the Amyloid Precursor Protein swe/Presenelin 1 dE9 (APP/PS1) mouse model of AD at 6 and 12 months of age (moa). Data independent acquisition (DIA), or Sequential Window Acquisition of all THeoretical fragment-ion (SWATH), was used for a quantitative label-free proteomics analysis. We first assessed the usefulness of a recently improved directDIA workflow as an alternative to conventional DIA data analysis using a project-specific spectral l"],"journal":["Cells"],"pubmed_title":["Age-Dependent Hippocampal Proteomics in the APP/PS1 Alzheimer Mouse Model: A Comparative Analysis with Classical SWATH/DIA and directDIA Approaches."],"pmcid":["PMC8304546"],"funding_grant_id":["733051076","733050811","733050106"],"pubmed_authors":["Li KW","Miedema SSM","Paliukhovich I","Koopmans F","van der Spek SJF","Gonzalez-Lozano MA","Smit AB"],"additional_accession":[]},"is_claimable":false,"name":"Age-Dependent Hippocampal Proteomics in the APP/PS1 Alzheimer Mouse Model: A Comparative Analysis with Classical SWATH/DIA and directDIA Approaches.","description":"Alzheimer's disease (AD) is the most common neurodegenerative disorder in the human population, for which there is currently no cure. The cause of AD is unknown; however, the toxic effects of amyloid-β (Aβ) are believed to play a role in its onset. To investigate this, we examined changes in global protein levels in a hippocampal synaptosome fraction of the Amyloid Precursor Protein swe/Presenelin 1 dE9 (APP/PS1) mouse model of AD at 6 and 12 months of age (moa). Data independent acquisition (DIA), or Sequential Window Acquisition of all THeoretical fragment-ion (SWATH), was used for a quantitative label-free proteomics analysis. We first assessed the usefulness of a recently improved directDIA workflow as an alternative to conventional DIA data analysis using a project-specific spectral l","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2026-04-08T08:58:11.352Z","creation":"2022-02-10T23:39:03.24Z"},"accession":"S-EPMC8304546","cross_references":{"pubmed":["34202490"],"doi":["10.3390/cells10071588"]}}