{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen Z"],"funding":["National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS","National Institute on Aging","National Science Foundation"],"pagination":["1542-1553.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8313648"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(11)"],"pubmed_abstract":["Peroxynitrite is a reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools that permit the selective, sensitive, and noninvasive detection of peroxynitrite are essential for understanding its pathophysiological functions. Here, we present pnGFP-Ultra, a high-performance, reaction-based, genetically encodable biosensor for imaging peroxynitrite in live cells. pnGFP-Ultra features a p-boronophenylalanine-modified chromophore as the sensing moiety and exhibits a remarkable ~110-fold fluorescence turn-on response toward peroxynitrite while displaying virtually no cross-reaction with other reactive oxygen/nitrogen species. To facilitate the expression of pnGFP-Ultra in mammalian cells, we engineered"],"journal":["Cell chemical biology"],"pubmed_title":["A high-performance genetically encoded fluorescent biosensor for imaging physiological peroxynitrite."],"pmcid":["PMC8313648"],"funding_grant_id":["3R01GM129291-02S2","CHE-1750660","R01 GM129291","R01GM118675","R01 GM118675"],"pubmed_authors":["Li X","Ai HW","Chen Z","Zhang S"],"additional_accession":[]},"is_claimable":false,"name":"A high-performance genetically encoded fluorescent biosensor for imaging physiological peroxynitrite.","description":"Peroxynitrite is a reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools that permit the selective, sensitive, and noninvasive detection of peroxynitrite are essential for understanding its pathophysiological functions. Here, we present pnGFP-Ultra, a high-performance, reaction-based, genetically encodable biosensor for imaging peroxynitrite in live cells. pnGFP-Ultra features a p-boronophenylalanine-modified chromophore as the sensing moiety and exhibits a remarkable ~110-fold fluorescence turn-on response toward peroxynitrite while displaying virtually no cross-reaction with other reactive oxygen/nitrogen species. To facilitate the expression of pnGFP-Ultra in mammalian cells, we engineered","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-18T12:57:06.37Z","creation":"2025-04-06T22:25:06.556Z"},"accession":"S-EPMC8313648","cross_references":{"pubmed":["33581056"],"doi":["10.1016/j.chembiol.2021.01.013"]}}