{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["42(4)"],"submitter":["Chen ZZ"],"pubmed_abstract":["Mutations of <i>PTEN-induced kinase I</i> (<i>PINK1</i>) cause early-onset Parkinson's disease (PD) with selective neurodegeneration in humans. However, current <i>PINK1</i> knockout mouse and pig models are unable to recapitulate the typical neurodegenerative phenotypes observed in PD patients. This suggests that generating <i>PINK1</i> disease models in non-human primates (NHPs) that are close to humans is essential to investigate the unique function of PINK1 in primate brains. Paired single guide RNA (sgRNA)/Cas9-D10A nickases and truncated sgRNA/Cas9, both of which can reduce off-target effects without compromising on-target editing, are two optimized strategies in the CRISPR/Cas9 system for establishing disease animal models. Here, we combined the two strategies and injected Cas9-D10A"],"journal":["Zoological research"],"pagination":["469-477"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8317192"],"repository":["biostudies-literature"],"pmcid":["PMC8317192"],"pubmed_authors":["Zhi DL","Yan L","Niu YY","Chen ZZ","Yang QY","Long CZ","Shen B","Wang JY","Kang Y","Gu XY"],"additional_accession":[]},"is_claimable":false,"name":null,"description":"Mutations of <i>PTEN-induced kinase I</i> (<i>PINK1</i>) cause early-onset Parkinson's disease (PD) with selective neurodegeneration in humans. However, current <i>PINK1</i> knockout mouse and pig models are unable to recapitulate the typical neurodegenerative phenotypes observed in PD patients. This suggests that generating <i>PINK1</i> disease models in non-human primates (NHPs) that are close to humans is essential to investigate the unique function of PINK1 in primate brains. Paired single guide RNA (sgRNA)/Cas9-D10A nickases and truncated sgRNA/Cas9, both of which can reduce off-target effects without compromising on-target editing, are two optimized strategies in the CRISPR/Cas9 system for establishing disease animal models. Here, we combined the two strategies and injected Cas9-D10A","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-19T20:42:17.115Z","creation":"2022-02-11T01:06:26.53Z"},"accession":"S-EPMC8317192","cross_references":{"pubmed":["34213093"],"doi":["10.24272/j.issn.2095-8137.2021.023"]}}