{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cheng H"],"funding":["National Cancer Institute","NCI NIH HHS"],"pagination":["113368"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8321683"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["190"],"pubmed_abstract":["D-2-hydroxyglutarate (D2HG) is over-produced as an oncometabolite due to mutations in isocitrate dehydrogenases (IDHs). Accumulation of D2HG can cause the dysfunction of many enzymes and genome-wide epigenetic alterations, which can promote oncogenesis. Quantification of D2HG at single-cell resolution can help understand the phenotypic signatures of IDH-mutant cancers and identify effective therapeutics. In this study, we developed an analytical method to detect D2HG levels in single cancer cells by adapting cascade enzymatic reactions on a resazurin-based fluorescence reporter. The resazurin probe was immobilized to the sensing surface via biotin-streptavidin interaction. This surface chemistry was rationally optimized to translate the D2HG levels to sensitive fluorescence readouts effici"],"journal":["Biosensors & bioelectronics"],"pubmed_title":["Single-cell profiling of D-2-hydroxyglutarate using surface-immobilized resazurin analogs."],"pmcid":["PMC8321683"],"funding_grant_id":["U01 CA217655","R03 CA227352","U54 CA199090"],"pubmed_authors":["Li Z","Guo Z","Mo L","Wei W","Xue M","Cheng H","Shao S"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell profiling of D-2-hydroxyglutarate using surface-immobilized resazurin analogs.","description":"D-2-hydroxyglutarate (D2HG) is over-produced as an oncometabolite due to mutations in isocitrate dehydrogenases (IDHs). Accumulation of D2HG can cause the dysfunction of many enzymes and genome-wide epigenetic alterations, which can promote oncogenesis. Quantification of D2HG at single-cell resolution can help understand the phenotypic signatures of IDH-mutant cancers and identify effective therapeutics. In this study, we developed an analytical method to detect D2HG levels in single cancer cells by adapting cascade enzymatic reactions on a resazurin-based fluorescence reporter. The resazurin probe was immobilized to the sensing surface via biotin-streptavidin interaction. This surface chemistry was rationally optimized to translate the D2HG levels to sensitive fluorescence readouts effici","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-05T20:11:54.636Z","creation":"2025-04-05T20:11:54.636Z"},"accession":"S-EPMC8321683","cross_references":{"pubmed":["34098361"],"doi":["10.1016/j.bios.2021.113368"]}}