{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Y"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NIA NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute on Aging","NHLBI NIH HHS","U.S. Department of Health & Human Services | NIH | National Cancer Institute","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute","NCI NIH HHS","NIGMS NIH HHS","NIH HHS"],"pagination":["4618"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8322156"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["The transcriptional co-activator and acetyltransferase p300 is required for fundamental cellular processes, including differentiation and growth. Here, we report that p300 forms phase separated condensates in the cell nucleus. The phase separation ability of p300 is regulated by autoacetylation and relies on its catalytic core components, including the histone acetyltransferase (HAT) domain, the autoinhibition loop, and bromodomain. p300 condensates sequester chromatin components, such as histone H3 tail and DNA, and are amplified through binding of p300 to the nucleosome. The catalytic HAT activity of p300 is decreased due to occlusion of the active site in the phase separated droplets, a large portion of which co-localizes with chromatin regions enriched in H3K27me3. Our findings suggest"],"journal":["Nature communications"],"pubmed_title":["Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity."],"pmcid":["PMC8322156"],"funding_grant_id":["R01 GM125195","HL151334","R01 CA252707","R01 HL151334","AG067664","R01 GM135286","R01 GM135671","R01 AG067664","K99 CA241301","R00 CA241301","R01 CA204020","CA252707","GM125195","GM135671","R56 AG067664","S10 OD025267"],"pubmed_authors":["Ren X","Liu J","Panne D","Ingersoll S","Zandian M","Shi X","Kutateladze TG","Ibrahim Z","Zhang Y","Brown K","Xuan H","Ebmeier CC","Lee T","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity.","description":"The transcriptional co-activator and acetyltransferase p300 is required for fundamental cellular processes, including differentiation and growth. Here, we report that p300 forms phase separated condensates in the cell nucleus. The phase separation ability of p300 is regulated by autoacetylation and relies on its catalytic core components, including the histone acetyltransferase (HAT) domain, the autoinhibition loop, and bromodomain. p300 condensates sequester chromatin components, such as histone H3 tail and DNA, and are amplified through binding of p300 to the nucleosome. The catalytic HAT activity of p300 is decreased due to occlusion of the active site in the phase separated droplets, a large portion of which co-localizes with chromatin regions enriched in H3K27me3. Our findings suggest","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2026-05-08T19:26:29.234Z","creation":"2022-02-11T04:18:13.206Z"},"accession":"S-EPMC8322156","cross_references":{"pubmed":["34326347"],"doi":["10.1038/s41467-021-24950-8"]}}