biostudies-literatureSajjad Haider MKing Saud University4384-4398https://www.ebi.ac.uk/biostudies/studies/S-EPMC8325032biostudies-literatureUnknown28(8)In the current study, we investigated the phytochemical and neuropharmacological potential of <i>Indigofera sessiliflora</i>, an indigenous least characterized plant widely distributed in deserted areas of Pakistan. The crude extract of the whole plant <i>Indigofera sessiliflora</i> (IS.CR) was preliminary tested <i>in-vitro</i> for the existence of polyphenol content, antioxidant and anticholinesterase potential followed by detailed chemical characterization through UHPLC-MS. Rats administered with different doses of IS.CR (100-300 mg/kg) for the duration of 4-weeks were behaviorally tested for anxiety and cognition followed by biochemical evaluation of dissected brain. The <i>in-silico</i> studies were employed to predict the blood-brain barrier crossing tendencies of secondary metabolitSaudi journal of biological sciencesChemical characterization and evaluation of the neuroprotective potential of <i>Indigofera sessiliflora through in-silico</i> studies and behavioral tests in scopolamine-induced memory compromised rats.PMC8325032RSP-2020/131Alasmari FMorales-Bayuelo AAlqahtani FJavaid SSajjad Haider MMuhammad Abdur Rahman HSiddique FFawad Rasool MKaya SMuhammad Muneeb Anjum SAshraf WSaleem HImran IfalseChemical characterization and evaluation of the neuroprotective potential of <i>Indigofera sessiliflora through in-silico</i> studies and behavioral tests in scopolamine-induced memory compromised rats.In the current study, we investigated the phytochemical and neuropharmacological potential of <i>Indigofera sessiliflora</i>, an indigenous least characterized plant widely distributed in deserted areas of Pakistan. The crude extract of the whole plant <i>Indigofera sessiliflora</i> (IS.CR) was preliminary tested <i>in-vitro</i> for the existence of polyphenol content, antioxidant and anticholinesterase potential followed by detailed chemical characterization through UHPLC-MS. Rats administered with different doses of IS.CR (100-300 mg/kg) for the duration of 4-weeks were behaviorally tested for anxiety and cognition followed by biochemical evaluation of dissected brain. The <i>in-silico</i> studies were employed to predict the blood-brain barrier crossing tendencies of secondary metabolit2021-01-01T00:00:00Z2021 Aug2025-04-20T01:06:50.974Z2022-02-11T05:15:54.264ZS-EPMC83250323435442310.1016/j.sjbs.2021.04.033