{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nakamura K"],"funding":["KAKENHI","AMED"],"pagination":["e11871"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8325423"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["Shiga toxin-producing <i>Escherichia coli</i> (STEC) is a major intestinal pathogen and causes serious gastrointestinal illness, which includes diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. The major virulence factors of STEC are Shiga toxins (Stx1 and Stx2), which belong to the AB-type toxin family. Among several subtypes of Stx1 and Stx2, the production of Stx2a is thought to be a risk factor for severe STEC infections, but Stx2a production levels vary markedly between STEC strains, even strains with the same serotype. Therefore, quantitative analyses of Stx2 production by STEC strains are important to understand the virulence potential of specific lineages or sublineages. In this study, we developed a novel Stx2 quantification method by utilizing homogen"],"journal":["PeerJ"],"pubmed_title":["Development of a homogeneous time-resolved FRET (HTRF) assay for the quantification of Shiga toxin 2 produced by <i>E. coli</i>."],"pmcid":["PMC8325423"],"funding_grant_id":["20fk0108065h0803 and 21fk0108611h0501","18K07116"],"pubmed_authors":["Ogura Y","Etoh Y","Nakamura K","Gotoh Y","Tokuda C","Taniguchi I","Hayashi T","Arimitsu H","Hamasaki M","Deguchi Y"],"additional_accession":[]},"is_claimable":false,"name":"Development of a homogeneous time-resolved FRET (HTRF) assay for the quantification of Shiga toxin 2 produced by <i>E. coli</i>.","description":"Shiga toxin-producing <i>Escherichia coli</i> (STEC) is a major intestinal pathogen and causes serious gastrointestinal illness, which includes diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. The major virulence factors of STEC are Shiga toxins (Stx1 and Stx2), which belong to the AB-type toxin family. Among several subtypes of Stx1 and Stx2, the production of Stx2a is thought to be a risk factor for severe STEC infections, but Stx2a production levels vary markedly between STEC strains, even strains with the same serotype. Therefore, quantitative analyses of Stx2 production by STEC strains are important to understand the virulence potential of specific lineages or sublineages. In this study, we developed a novel Stx2 quantification method by utilizing homogen","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-09T00:39:53.275Z","creation":"2022-02-11T08:09:01.785Z"},"accession":"S-EPMC8325423","cross_references":{"pubmed":["34395095"],"doi":["10.7717/peerj.11871"]}}