{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang X"],"funding":["Eunice Kennedy Shriver National Institute of Child Health and Human Development","NICHD NIH HHS","University of Georgia","National Science Foundation"],"pagination":["jcs256297"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8325960"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["134(14)"],"pubmed_abstract":["Oocyte-specific knockdown of pericentrin (PCNT) in transgenic (Tg) mice disrupts acentriolar microtubule-organizing center (aMTOC) formation, leading to spindle instability and error-prone meiotic division. Here, we show that PCNT-depleted oocytes lack phosphorylated Aurora A (pAURKA) at spindle poles, while overall levels are unaltered. To test aMTOC-associated AURKA function, metaphase II (MII) control (WT) and Tg oocytes were briefly exposed to a specific AURKA inhibitor (MLN8237). Similar defects were observed in Tg and MLN8237-treated WT oocytes, including altered spindle structure, increased chromosome misalignment and impaired microtubule regrowth. Yet, AURKA inhibition had a limited effect on Tg oocytes, revealing a critical role for aMTOC-associated AURKA in regulating spindle sta"],"journal":["Journal of cell science"],"pubmed_title":["Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes."],"pmcid":["PMC8325960"],"funding_grant_id":["HD093383","HD092857","R56 HD093383","R03 HD092857"],"pubmed_authors":["Baumann C","De La Fuente R","Wang X","Viveiros MM"],"additional_accession":[]},"is_claimable":false,"name":"Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes.","description":"Oocyte-specific knockdown of pericentrin (PCNT) in transgenic (Tg) mice disrupts acentriolar microtubule-organizing center (aMTOC) formation, leading to spindle instability and error-prone meiotic division. Here, we show that PCNT-depleted oocytes lack phosphorylated Aurora A (pAURKA) at spindle poles, while overall levels are unaltered. To test aMTOC-associated AURKA function, metaphase II (MII) control (WT) and Tg oocytes were briefly exposed to a specific AURKA inhibitor (MLN8237). Similar defects were observed in Tg and MLN8237-treated WT oocytes, including altered spindle structure, increased chromosome misalignment and impaired microtubule regrowth. Yet, AURKA inhibition had a limited effect on Tg oocytes, revealing a critical role for aMTOC-associated AURKA in regulating spindle sta","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-19T20:45:18.217Z","creation":"2025-02-19T00:25:42.004Z"},"accession":"S-EPMC8325960","cross_references":{"pubmed":["34152366"],"doi":["10.1242/jcs.256297"]}}