biostudies-literatureUnknown9Li Y<h4>Background</h4>Quiescin Q6 sulfhydryl oxidase 2 (QSOX2), an enzyme that can be directly secreted into the extracellular space, is known to be associated with oxidative protein folding. However, whether <i>QSOX2</i> is abnormally expressed in non-small cell lung cancer (NSCLC) and its role in tumor growth remains unclear.<h4>Methods</h4>Real-time quantitative PCR (qPCR), immunohistochemistry (IHC), bioinformatics analyses were applied to analyze the expression pattern and prognostic significance of QSOX2 in NSCLC. Xenografts model, enzyme-linked immunosorbent assays (ELISA), western blot analysis (WB), and IHC were preformed to examine <i>in vivo</i> tumor suppression and intracellular and extracellular expression of QSOX2. Flow cytometry, WB and qPCR analyses were used to elucidate theFrontiers in cell and developmental biology688798https://www.ebi.ac.uk/biostudies/studies/S-EPMC8326667biostudies-literatureQSOX2 Is an E2F1 Target Gene and a Novel Serum Biomarker for Monitoring Tumor Growth and Predicting Survival in Advanced NSCLC.PMC8326667Liu HXiong JLi YLiu MZhang ZShi CDeng LZhai ZWang YZhang CfalseQSOX2 Is an E2F1 Target Gene and a Novel Serum Biomarker for Monitoring Tumor Growth and Predicting Survival in Advanced NSCLC.<h4>Background</h4>Quiescin Q6 sulfhydryl oxidase 2 (QSOX2), an enzyme that can be directly secreted into the extracellular space, is known to be associated with oxidative protein folding. However, whether <i>QSOX2</i> is abnormally expressed in non-small cell lung cancer (NSCLC) and its role in tumor growth remains unclear.<h4>Methods</h4>Real-time quantitative PCR (qPCR), immunohistochemistry (IHC), bioinformatics analyses were applied to analyze the expression pattern and prognostic significance of QSOX2 in NSCLC. Xenografts model, enzyme-linked immunosorbent assays (ELISA), western blot analysis (WB), and IHC were preformed to examine <i>in vivo</i> tumor suppression and intracellular and extracellular expression of QSOX2. Flow cytometry, WB and qPCR analyses were used to elucidate the2021-01-01T00:00:00Z20212026-05-08T12:39:19.916Z2022-02-11T04:28:47.562ZS-EPMC83266673435018110.3389/fcell.2021.688798