{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(16)"],"submitter":["Guerneve CL"],"pubmed_abstract":["We used <i>in vivo</i> and <i>in vitro</i> phosphorus-31 nuclear magnetic resonance (<sup>31</sup>P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus <i>Hebeloma cylindrosporum</i> in response to root signals originating from host (<i>Pinus pinaster</i>) or non-host (<i>Zea mays</i>) plants. A device was developed for the <i>in vivo</i> studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The <i>in vitro</i> studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium)."],"journal":["Bio-protocol"],"pagination":["e2973"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8328686"],"repository":["biostudies-literature"],"pubmed_title":["<i>In vivo</i> and <i>in vitro</i> <sup>31</sup>P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in <i>Hebeloma cylindrosporum</i> in Response to Plant Roots Signals."],"pmcid":["PMC8328686"],"pubmed_authors":["Guerneve CL","Quiquampoix H","Trives-Segura C","Staunton S","Amenc LK","Becquer A","Torres-Aquino M","Plassard C"],"additional_accession":[]},"is_claimable":false,"name":"<i>In vivo</i> and <i>in vitro</i> <sup>31</sup>P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in <i>Hebeloma cylindrosporum</i> in Response to Plant Roots Signals.","description":"We used <i>in vivo</i> and <i>in vitro</i> phosphorus-31 nuclear magnetic resonance (<sup>31</sup>P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus <i>Hebeloma cylindrosporum</i> in response to root signals originating from host (<i>Pinus pinaster</i>) or non-host (<i>Zea mays</i>) plants. A device was developed for the <i>in vivo</i> studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The <i>in vitro</i> studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium).","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Aug","modification":"2026-07-09T11:25:52.265Z","creation":"2026-07-09T10:48:16.247Z"},"accession":"S-EPMC8328686","cross_references":{"pubmed":["34395775"],"doi":["10.21769/BioProtoc.2973","10.21769/bioprotoc.2973"]}}