biostudies-literatureUnknown8(16)Guerneve CLWe used <i>in vivo</i> and <i>in vitro</i> phosphorus-31 nuclear magnetic resonance (³¹P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus <i>Hebeloma cylindrosporum</i> in response to root signals originating from host (<i>Pinus pinaster</i>) or non-host (<i>Zea mays</i>) plants. A device was developed for the <i>in vivo</i> studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The <i>in vitro</i> studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium).Bio-protocole2973https://www.ebi.ac.uk/biostudies/studies/S-EPMC8328686biostudies-literature<i>In vivo</i> and <i>in vitro</i> ³¹P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in <i>Hebeloma cylindrosporum</i> in Response to Plant Roots Signals.PMC8328686Guerneve CLQuiquampoix HTrives-Segura CStaunton SAmenc LKBecquer ATorres-Aquino MPlassard Cfalse<i>In vivo</i> and <i>in vitro</i> ³¹P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in <i>Hebeloma cylindrosporum</i> in Response to Plant Roots Signals.We used <i>in vivo</i> and <i>in vitro</i> phosphorus-31 nuclear magnetic resonance (³¹P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus <i>Hebeloma cylindrosporum</i> in response to root signals originating from host (<i>Pinus pinaster</i>) or non-host (<i>Zea mays</i>) plants. A device was developed for the <i>in vivo</i> studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The <i>in vitro</i> studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium).2018-01-01T00:00:00Z2018 Aug2026-07-09T11:25:52.265Z2026-07-09T10:48:16.247ZS-EPMC83286863439577510.21769/BioProtoc.297310.21769/bioprotoc.2973