{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["71(2)"],"submitter":["Shibaya T"],"pubmed_abstract":["In carrot (<i>Daucus carota</i> L.), the taproot colors orange, yellow and white are determined mostly by the <i>Y</i>, <i>Y2</i>, and <i>Or</i> loci. One of the most severe issues in carrot seed production is contamination by wild white carrot. To evaluate the contamination ratio, easily detectable DNA markers for white carrot are desired. To develop PCR-based DNA markers for the <i>Y2</i> locus, we have re-sequenced two orange-colored carrot cultivars at our company (Fujii Seed, Japan), as well as six white- and one light-orange-colored carrots that contaminated our seed products. Within the candidate region previously reported for the <i>Y2</i> locus, only one DNA marker, Y2_7, clearly distinguished white carrots from orange ones in the re-sequenced samples. The Y2_7 marker was further "],"journal":["Breeding science"],"pagination":["201-207"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8329877"],"repository":["biostudies-literature"],"pubmed_title":["Development of PCR-based DNA marker for detection of white carrot contamination caused by <i>Y2</i> locus."],"pmcid":["PMC8329877"],"pubmed_authors":["Kuroda C","Nakayama S","Isobe S","Minami C","Shibaya T","Obara A","Fujii T"],"additional_accession":[]},"is_claimable":false,"name":"Development of PCR-based DNA marker for detection of white carrot contamination caused by <i>Y2</i> locus.","description":"In carrot (<i>Daucus carota</i> L.), the taproot colors orange, yellow and white are determined mostly by the <i>Y</i>, <i>Y2</i>, and <i>Or</i> loci. One of the most severe issues in carrot seed production is contamination by wild white carrot. To evaluate the contamination ratio, easily detectable DNA markers for white carrot are desired. To develop PCR-based DNA markers for the <i>Y2</i> locus, we have re-sequenced two orange-colored carrot cultivars at our company (Fujii Seed, Japan), as well as six white- and one light-orange-colored carrots that contaminated our seed products. Within the candidate region previously reported for the <i>Y2</i> locus, only one DNA marker, Y2_7, clearly distinguished white carrots from orange ones in the re-sequenced samples. The Y2_7 marker was further ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-21T17:40:11.625Z","creation":"2022-02-11T06:06:50.659Z"},"accession":"S-EPMC8329877","cross_references":{"pubmed":["34377068"],"doi":["10.1270/jsbbs.20120"]}}