biostudies-literatureUnknown12Zhou W<i>Candida auris</i> emerged as a pathogenic species of fungus that causes severe and invasive outbreaks worldwide. The fungus exhibits high intrinsic resistance rates to various first-line antifungals, and the underlying molecular mechanism responsible for its multidrug resistance is still unclear. In this study, a transcriptomic analysis was performed between two <i>C. auris</i> isolates that exhibited different anti-drug patterns by RNA-sequencing, namely, CX1 (anti-drug sensitive) and CX2 (resistant). Transcriptomic analysis results revealed 541 upregulated and 453 downregulated genes in the resistant <i>C. auris</i> strain compared with the susceptible strain. In addition, our findings highlight the presence of potential differentially expressed genes (DEGs), which may play a role in drug resistance, including genes involved in ergosterol and efflux pump biosynthesis such as <i>SNQ2</i>, <i>CDR4</i>, <i>ARB1</i>, <i>MDR1</i>, <i>MRR1</i>, and <i>ERG</i> genes. We also found that Hsp related genes were upregulated for expression in the anti-drug-resistant strain. Biofilm formation and growth conditions were also compared between the two isolates. Our study provides novel clues for future studies in terms of understanding multidrug resistance mechanisms of <i>C. auris</i> strains.Frontiers in microbiology708009https://www.ebi.ac.uk/biostudies/studies/S-EPMC8330549biostudies-literatureA Comparative Transcriptome Between Anti-drug Sensitive and Resistant <i>Candida auris</i> in China.PMC8330549Yang XLi XHuang XQiao DZhou WLin YJiang SYan WLi NfalseA Comparative Transcriptome Between Anti-drug Sensitive and Resistant <i>Candida auris</i> in China.<i>Candida auris</i> emerged as a pathogenic species of fungus that causes severe and invasive outbreaks worldwide. The fungus exhibits high intrinsic resistance rates to various first-line antifungals, and the underlying molecular mechanism responsible for its multidrug resistance is still unclear. In this study, a transcriptomic analysis was performed between two <i>C. auris</i> isolates that exhibited different anti-drug patterns by RNA-sequencing, namely, CX1 (anti-drug sensitive) and CX2 (resistant). Transcriptomic analysis results revealed 541 upregulated and 453 downregulated genes in the resistant <i>C. auris</i> strain compared with the susceptible strain. In addition, our findings highlight the presence of potential differentially expressed genes (DEGs), which may play a role in drug resistance, including genes involved in ergosterol and efflux pump biosynthesis such as <i>SNQ2</i>, <i>CDR4</i>, <i>ARB1</i>, <i>MDR1</i>, <i>MRR1</i>, and <i>ERG</i> genes. We also found that Hsp related genes were upregulated for expression in the anti-drug-resistant strain. Biofilm formation and growth conditions were also compared between the two isolates. Our study provides novel clues for future studies in terms of understanding multidrug resistance mechanisms of <i>C. auris</i> strains.2021-01-01T00:00:00Z20212024-11-19T20:48:02.959Z2022-02-11T05:13:16.741ZS-EPMC83305493435469510.3389/fmicb.2021.708009