biostudies-literatureUnknown11(1)De Blasi RAWe developed a method for measuring in vivo venular volumes and the mean systemic filling pressure in the limbs using near-infrared spectroscopy (NIRS). We aimed to validate the NIRS methodology by comparing two independent methods of calculation based on different physiological approaches. Pressure-volumes (P-V) curves were recorded following graded venous occlusion on the forearm. Values from a P-V curves analysis model (method 1) were compared with data derived from a resistor-capacitance calculation model (method 2) based on arterial pressure and venous compliance. We tested these methods on 10 healthy participants at rest and during exercise and on 6 severely ill patients. Results from method 1 were comparable with those calculated by method 2. Venular volumes calculated using method 1 correlated linearly with those calculated using method 2 both in participants (R² = 0.98) and in patients (R² = 0.94). A good agreement between methods was shown with few values out of the range of ± 1.96 standard deviation. Our findings added mathematical consistency for the NIRS methodology validation in the venular P-V assessment with no flow interruption. Further research will be required to confirm the relevance of the methodology in the clinical setting.Scientific reports15844https://www.ebi.ac.uk/biostudies/studies/S-EPMC8338930biostudies-literatureValidation of the mean systemic filling pressure assessment with preserved arterial blood flow by comparing two methods of calculation.PMC8338930De Blasi RAFinazzi SfalseValidation of the mean systemic filling pressure assessment with preserved arterial blood flow by comparing two methods of calculation.We developed a method for measuring in vivo venular volumes and the mean systemic filling pressure in the limbs using near-infrared spectroscopy (NIRS). We aimed to validate the NIRS methodology by comparing two independent methods of calculation based on different physiological approaches. Pressure-volumes (P-V) curves were recorded following graded venous occlusion on the forearm. Values from a P-V curves analysis model (method 1) were compared with data derived from a resistor-capacitance calculation model (method 2) based on arterial pressure and venous compliance. We tested these methods on 10 healthy participants at rest and during exercise and on 6 severely ill patients. Results from method 1 were comparable with those calculated by method 2. Venular volumes calculated using method 1 correlated linearly with those calculated using method 2 both in participants (R² = 0.98) and in patients (R² = 0.94). A good agreement between methods was shown with few values out of the range of ± 1.96 standard deviation. Our findings added mathematical consistency for the NIRS methodology validation in the venular P-V assessment with no flow interruption. Further research will be required to confirm the relevance of the methodology in the clinical setting.2021-01-01T00:00:00Z2021 Aug2024-02-15T09:01:27.645Z2022-02-11T04:14:01.872ZS-EPMC83389303434921910.1038/s41598-021-95350-7