{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(7)"],"submitter":["Zou Y"],"pubmed_abstract":["<h4>Objective</h4>Mast cells-derived extracellular vesicles (EVs) play vital roles in various physiological and pathophysiological conditions. However, the cargoes of mast cells-derived EVs in asthma have not been established. Here, we set to identify the role of microRNA-21 (miRNA-21) from mast cells-derived EVs in ozone- and lipopolysaccharide (LPS)-induced mouse airway epithelial cells (MIC-iCell-a006 cells) and asthmatic mice.<h4>Methods</h4>After ozone or LPS treatment, MIC-iCell-a006 cells were subjected to a microarray analysis to screen differentially expressed miRNAs, and then co-cultured with EVs. miR-21 was silenced in cells, followed by CCK-8, scratch, and Transwell assays. Mice were challenged with ovalbumin, and antioxidant enzymes and inflammatory cell infiltration were asse"],"journal":["American journal of translational research"],"pagination":["7475-7491"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8340267"],"repository":["biostudies-literature"],"pubmed_title":["MicroRNA-21 released from mast cells-derived extracellular vesicles drives asthma in mice by potentiating airway inflammation and oxidative stress."],"pmcid":["PMC8340267"],"pubmed_authors":["Zou Y","Zhou Q","Zhang Y"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-21 released from mast cells-derived extracellular vesicles drives asthma in mice by potentiating airway inflammation and oxidative stress.","description":"<h4>Objective</h4>Mast cells-derived extracellular vesicles (EVs) play vital roles in various physiological and pathophysiological conditions. However, the cargoes of mast cells-derived EVs in asthma have not been established. Here, we set to identify the role of microRNA-21 (miRNA-21) from mast cells-derived EVs in ozone- and lipopolysaccharide (LPS)-induced mouse airway epithelial cells (MIC-iCell-a006 cells) and asthmatic mice.<h4>Methods</h4>After ozone or LPS treatment, MIC-iCell-a006 cells were subjected to a microarray analysis to screen differentially expressed miRNAs, and then co-cultured with EVs. miR-21 was silenced in cells, followed by CCK-8, scratch, and Transwell assays. Mice were challenged with ovalbumin, and antioxidant enzymes and inflammatory cell infiltration were asse","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-06-01T03:30:08.682Z","creation":"2025-06-01T03:30:08.682Z"},"accession":"S-EPMC8340267","cross_references":{"pubmed":["34377230"]}}