{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Long MJC"],"funding":["Swiss National Science Foundation","NIH Office of the Director","NIGMS NIH HHS"],"pagination":["42-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8341840"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1(2)"],"pubmed_abstract":["Transient associations between numerous organelles-<i>e.g.</i>, the endoplasmic reticulum and the mitochondria-forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical-biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical-biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology."],"journal":["RSC chemical biology"],"pubmed_title":["Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities."],"pmcid":["PMC8341840"],"funding_grant_id":["190192","DP2 GM114850","184729","NIH 1DP2GM114850"],"pubmed_authors":["Aye Y","Zhao Y","Long MJC"],"additional_accession":[]},"is_claimable":false,"name":"Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities.","description":"Transient associations between numerous organelles-<i>e.g.</i>, the endoplasmic reticulum and the mitochondria-forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical-biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical-biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2025-04-04T19:42:42.793Z","creation":"2022-02-11T10:03:53.574Z"},"accession":"S-EPMC8341840","cross_references":{"pubmed":["34458747"],"doi":["10.1039/d0cb00041h"]}}