biostudies-literatureXia YNIAID NIH HHSNIMH NIH HHSNational Institute of Mental HealthNIGMS NIH HHS133-142https://www.ebi.ac.uk/biostudies/studies/S-EPMC8342043biostudies-literatureUnknown75(2-3)A unique aspect of NMR is its capacity to provide integrated insight into both the structure and intrinsic dynamics of biomolecules. Chemical exchange phenomena that often serve as probes of dynamic processes in biological macromolecules can be quantitatively investigated with chemical exchange saturation transfer (CEST) experiments. ²H-decoupling sidebands, however, always occur in the profiles of ¹³CHD₂ ¹³C-CEST experiments when using the simple CW (continuous wave) method, which may obscure the detection of minor dips of excited states. Traditionally, these sidebands are manually eliminated from the profiles before data analysis by removing experimental points in the range of ²H-decoupling field strength ±50 Hz away from the major dJournal of biomolecular NMRRemoval of ²H-decoupling sidebands in ¹³CHD₂ ¹³C-CEST profiles.PMC8342043R35 GM122462R01 AI094623R01 AI121858Xia YKalodimos CGYuwen TLiu AfalseRemoval of ²H-decoupling sidebands in ¹³CHD₂ ¹³C-CEST profiles.A unique aspect of NMR is its capacity to provide integrated insight into both the structure and intrinsic dynamics of biomolecules. Chemical exchange phenomena that often serve as probes of dynamic processes in biological macromolecules can be quantitatively investigated with chemical exchange saturation transfer (CEST) experiments. ²H-decoupling sidebands, however, always occur in the profiles of ¹³CHD₂ ¹³C-CEST experiments when using the simple CW (continuous wave) method, which may obscure the detection of minor dips of excited states. Traditionally, these sidebands are manually eliminated from the profiles before data analysis by removing experimental points in the range of ²H-decoupling field strength ±50 Hz away from the major d2021-01-01T00:00:00Z2021 Mar2025-04-05T10:03:44.123Z2025-04-05T10:03:44.123ZS-EPMC83420433374506810.1007/s10858-021-00362-0