{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nasti RA"],"funding":["National Institutes of Health","NIGMS NIH HHS"],"pagination":["621710"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8344638"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2"],"pubmed_abstract":["The production of transgenic or gene edited plants requires considerable time and effort. It is of value to know at the onset of a project whether the transgenes or gene editing reagents are functioning as predicted. To test molecular reagents transiently, we implemented an improved, <i>Agrobacterium tumefaciens</i>-based co-culture method called Fast-TrACC (Fast Treated Agrobacterium Co-Culture). Fast-TrACC delivers reagents to seedlings, allowing high throughput, and uses a luciferase reporter to monitor and calibrate the efficiency of reagent delivery. We demonstrate the use of Fast-TrACC in multiple solanaceous species and apply the method to test promoter activity and the effectiveness of gene editing reagents."],"journal":["Frontiers in genome editing"],"pubmed_title":["Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells."],"pmcid":["PMC8344638"],"funding_grant_id":["T32 GM008347"],"pubmed_authors":["Nasti RA","Voytas DF","Vollbrecht M","Maher MF","Zinselmeier MH"],"additional_accession":[]},"is_claimable":false,"name":"Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells.","description":"The production of transgenic or gene edited plants requires considerable time and effort. It is of value to know at the onset of a project whether the transgenes or gene editing reagents are functioning as predicted. To test molecular reagents transiently, we implemented an improved, <i>Agrobacterium tumefaciens</i>-based co-culture method called Fast-TrACC (Fast Treated Agrobacterium Co-Culture). Fast-TrACC delivers reagents to seedlings, allowing high throughput, and uses a luciferase reporter to monitor and calibrate the efficiency of reagent delivery. We demonstrate the use of Fast-TrACC in multiple solanaceous species and apply the method to test promoter activity and the effectiveness of gene editing reagents.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-08-30T03:08:01.153Z","creation":"2024-12-03T19:29:33.585Z"},"accession":"S-EPMC8344638","cross_references":{"pubmed":["34368798"],"doi":["10.3389/fgeed.2020.621710"]}}