{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang K"],"funding":["National Institutes of Health","NIGMS NIH HHS"],"pagination":["100687"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8348308"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(3)"],"pubmed_abstract":["Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to assess the potency and specificity of interventions targeting CPT-1. Strengths of the approach include ease, speed, and breadth of analysis, whereas drawbacks include loss of physiological regulation in reductionist systems and indirect assessment of CPT-1 enzymatic activity. For complete details on the use and execution of this protocol, please refer to Divakaruni et al. (2018)."],"journal":["STAR protocols"],"pubmed_title":["Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria."],"pmcid":["PMC8348308"],"funding_grant_id":["R35GM138003","P30DK063491","R35 GM138003"],"pubmed_authors":["Doan MT","Yang K","Stiles L","Divakaruni AS"],"additional_accession":[]},"is_claimable":false,"name":"Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria.","description":"Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to assess the potency and specificity of interventions targeting CPT-1. Strengths of the approach include ease, speed, and breadth of analysis, whereas drawbacks include loss of physiological regulation in reductionist systems and indirect assessment of CPT-1 enzymatic activity. For complete details on the use and execution of this protocol, please refer to Divakaruni et al. (2018).","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-05T08:50:24.948Z","creation":"2025-02-19T01:15:59.243Z"},"accession":"S-EPMC8348308","cross_references":{"pubmed":["34401773"],"doi":["10.1016/j.xpro.2021.100687"]}}