biostudies-literaturePatel MNIDCD NIH HHSNIHNIGMS NIH HHSNew Jersey Commission on Spinal Cord Research2469-2482https://www.ebi.ac.uk/biostudies/studies/S-EPMC8353206biostudies-literatureUnknown29(8)Promoting residential cells, particularly endogenous neural stem and progenitor cells (NSPCs), for tissue regeneration represents a potential strategy for the treatment of spinal cord injury (SCI). However, adult NSPCs differentiate mainly into glial cells and contribute to glial scar formation at the site of injury. Gsx1 is known to regulate the generation of excitatory and inhibitory interneurons during embryonic development of the spinal cord. In this study, we show that lentivirus-mediated expression of Gsx1 increases the number of NSPCs in a mouse model of lateral hemisection SCI during the acute stage. Subsequently, Gsx1 expression increases the generation of glutamatergic and cholinergic interneurons and decreases the generation of GABAergic interneurons in the chronic stage of SCI.Molecular therapy : the journal of the American Society of Gene TherapyGsx1 promotes locomotor functional recovery after spinal cord injury.PMC8353206R25 GM055145T32 GM139804R01 DC016612T32 GM135141T32 GM008339Anderson JLi YLyu YLCastro-Pedrido SLee KBFinkel ZCai LPatel MLei SSkinner REsteban FRodriguez BfalseGsx1 promotes locomotor functional recovery after spinal cord injury.Promoting residential cells, particularly endogenous neural stem and progenitor cells (NSPCs), for tissue regeneration represents a potential strategy for the treatment of spinal cord injury (SCI). However, adult NSPCs differentiate mainly into glial cells and contribute to glial scar formation at the site of injury. Gsx1 is known to regulate the generation of excitatory and inhibitory interneurons during embryonic development of the spinal cord. In this study, we show that lentivirus-mediated expression of Gsx1 increases the number of NSPCs in a mouse model of lateral hemisection SCI during the acute stage. Subsequently, Gsx1 expression increases the generation of glutamatergic and cholinergic interneurons and decreases the generation of GABAergic interneurons in the chronic stage of SCI.2021-01-01T00:00:00Z2021 Aug2026-05-08T11:19:33.963Z2025-04-04T09:30:44.495ZS-EPMC83532063389532310.1016/j.ymthe.2021.04.027