{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Neuman SD"],"funding":["National Institutes of Health","NIGMS NIH HHS","NIH HHS"],"pagination":["jcs259037"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8353522"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["134(15)"],"pubmed_abstract":["Regulated exocytosis is an essential process whereby specific cargo proteins are secreted in a stimulus-dependent manner. Cargo-containing secretory granules are synthesized in the trans-Golgi network (TGN); after budding from the TGN, granules undergo modifications, including an increase in size. These changes occur during a poorly understood process called secretory granule maturation. Here, we leverage the Drosophila larval salivary glands as a model to characterize a novel role for Rab GTPases during granule maturation. We find that secretory granules increase in size ∼300-fold between biogenesis and release, and loss of Rab1 or Rab11 reduces granule size. Surprisingly, we find that Rab1 and Rab11 localize to secretory granule membranes. Rab11 associates with granule membranes througho"],"journal":["Journal of cell science"],"pubmed_title":["A novel function for Rab1 and Rab11 during secretory granule maturation."],"pmcid":["PMC8353522"],"funding_grant_id":["GM123204","R01 GM123204","P40 OD018537"],"pubmed_authors":["Bashirullah A","Neuman SD","Lee AR","Selegue JE","Cavanagh AT"],"additional_accession":[]},"is_claimable":false,"name":"A novel function for Rab1 and Rab11 during secretory granule maturation.","description":"Regulated exocytosis is an essential process whereby specific cargo proteins are secreted in a stimulus-dependent manner. Cargo-containing secretory granules are synthesized in the trans-Golgi network (TGN); after budding from the TGN, granules undergo modifications, including an increase in size. These changes occur during a poorly understood process called secretory granule maturation. Here, we leverage the Drosophila larval salivary glands as a model to characterize a novel role for Rab GTPases during granule maturation. We find that secretory granules increase in size ∼300-fold between biogenesis and release, and loss of Rab1 or Rab11 reduces granule size. Surprisingly, we find that Rab1 and Rab11 localize to secretory granule membranes. Rab11 associates with granule membranes througho","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2025-04-22T10:01:03.314Z","creation":"2025-04-05T23:22:28.1Z"},"accession":"S-EPMC8353522","cross_references":{"pubmed":["34342349"],"doi":["10.1242/jcs.259037"]}}