{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ferkowicz MJ"],"funding":["NIDDK NIH HHS"],"pagination":["661-676"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8363780"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["101(5)"],"pubmed_abstract":["The advent of personalized medicine has driven the development of novel approaches for obtaining detailed cellular and molecular information from clinical tissue samples. Tissue cytometry is a promising new technique that can be used to enumerate and characterize each cell in a tissue and, unlike flow cytometry and other single-cell techniques, does so in the context of the intact tissue, preserving spatial information that is frequently crucial to understanding a cell's physiology, function, and behavior. However, the wide-scale adoption of tissue cytometry as a research tool has been limited by the fact that published examples utilize specialized techniques that are beyond the capabilities of most laboratories. Here we describe a complete and accessible pipeline, including methods of sam"],"journal":["Laboratory investigation; a journal of technical methods and pathology"],"pubmed_title":["Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline."],"pmcid":["PMC8363780"],"funding_grant_id":["UH3 DK114923","UG3 DK114923","K08 DK107864","P30 DK079312"],"pubmed_authors":["Mcmahon G","Williams M","Zhang G","Cooperman L","Otto E","Joanes M","Wright L","Zhang K","Williams K","Sanchez F","Appelbaum P","Pasa-Tolic L","Jefferson N","Sims-Lucas S","Sutton T","Wilson FP","Kiryluk K","Kermani A","Barwinska D","Oliver GH","Jain S","Koewler R","Moe O","Lake B","D'agati V","Villalobos P","He O","Rosas S","Carson J","Hacohen N","Goltsev Y","Sambandam K","Eadon M","Saul J","Lee S","Stefanick M","Parikh S","Velickovic D","Tublin M","Balis U","Miller T","Drager Z","Ugochukwu U","Kidney Precision Medicine Project","Fullerton M","Lecker S","Hodgin J","Randhawa P","Parikh C","Winfree S","Ashkar T","Cheng YH","Poggio E","Kelly K","Hall D","Shankland S","Murugan R","Schroeder A","Dagher P","Torrealba J","Anderton C","Tuttle K","Boer I","Blank K","He CJ","Khochare S","Schaub J","Dighe A","Knight R","Ferkowicz M","Kellum J","Roberts G","Eadon MT","Herlitz L","Rosengart M","Dowd F","Azeloglu E","Stutzke EC","Dobi D","Aulisio M","O'toole J","Menez S","Nolan G","Mcclelland R","Wang N","Dagher PC","Alpers C","Alexandrov T","Kamocka MM","Elder M","Moledina D","Sutton TA","Kelly KJ","Park H","Laszik Z","Phillips CL","Robert T","Lu C","Hoover P","Sharma K","Bebiak J","Waikar S","Dunn K","Barasch J","Weng C","Dunn KW","Kretzler M","Gaut J","Stillman I","Pinkeney R","Palevsky P","Mooney S","Ferkowicz MJ","Mehl K","Himmelfarb J","Barisoni L","Sedor J","Shang NS","Kruth M","Troyanskaya O","Mariani L","Steck B","Menon R","Luciano R","Rosenberg A","Iyengar R","Campbell T","Sealfon R","Bomback A","Slack A","Shpigel A","Miegs L","Vazquez M","Ardayfio J","Hayashi L","Weins A","Sabo AR","Brown K","Vijayan A","El-Achkar TM","Campbell C","Stull S"],"additional_accession":[]},"is_claimable":false,"name":"Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline.","description":"The advent of personalized medicine has driven the development of novel approaches for obtaining detailed cellular and molecular information from clinical tissue samples. Tissue cytometry is a promising new technique that can be used to enumerate and characterize each cell in a tissue and, unlike flow cytometry and other single-cell techniques, does so in the context of the intact tissue, preserving spatial information that is frequently crucial to understanding a cell's physiology, function, and behavior. However, the wide-scale adoption of tissue cytometry as a research tool has been limited by the fact that published examples utilize specialized techniques that are beyond the capabilities of most laboratories. Here we describe a complete and accessible pipeline, including methods of sam","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2025-04-05T10:04:00.08Z","creation":"2025-04-05T10:04:00.08Z"},"accession":"S-EPMC8363780","cross_references":{"pubmed":["33408350"],"doi":["10.1038/s41374-020-00518-w"]}}