{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Kealey JT"],"pubmed_abstract":["Lichen-forming fungi produce a variety of secondary metabolites including bioactive polyketides. Advances in DNA and RNA sequencing have led to a growing database of new lichen gene clusters encoding polyketide synthases (PKS) and associated ancillary activities. Definitive assignment of a PKS gene to a metabolic product has been challenging in the lichen field due to a lack of established gene knockout or heterologous gene expression systems. Here, we report the reconstitution of a non-reducing PKS gene from the lichen <i>Pseudevernia furfuracea</i> and successful heterologous expression of the synthetic lichen PKS gene in engineered <i>Saccharomyces cerevisiae</i>. We show that <i>P. furfuracea</i> PFUR17_02294 produces lecanoric acid, the depside dimer of orsellinic acid, at 360 mg/L in small-scale yeast cultures. Our results unequivocally identify PFUR17_02294 as a lecanoric acid synthase and establish that a single lichen PKS synthesizes two phenolic rings and joins them by an ester linkage to form the depside product."],"journal":["Metabolic engineering communications"],"pagination":["e00172"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8365352"],"repository":["biostudies-literature"],"pubmed_title":["Identification of a lichen depside polyketide synthase gene by heterologous expression in <i>Saccharomyces cerevisiae</i>."],"pmcid":["PMC8365352"],"pubmed_authors":["Kealey JT","Craig JP","Barr PJ"],"additional_accession":[]},"is_claimable":false,"name":"Identification of a lichen depside polyketide synthase gene by heterologous expression in <i>Saccharomyces cerevisiae</i>.","description":"Lichen-forming fungi produce a variety of secondary metabolites including bioactive polyketides. Advances in DNA and RNA sequencing have led to a growing database of new lichen gene clusters encoding polyketide synthases (PKS) and associated ancillary activities. Definitive assignment of a PKS gene to a metabolic product has been challenging in the lichen field due to a lack of established gene knockout or heterologous gene expression systems. Here, we report the reconstitution of a non-reducing PKS gene from the lichen <i>Pseudevernia furfuracea</i> and successful heterologous expression of the synthetic lichen PKS gene in engineered <i>Saccharomyces cerevisiae</i>. We show that <i>P. furfuracea</i> PFUR17_02294 produces lecanoric acid, the depside dimer of orsellinic acid, at 360 mg/L in small-scale yeast cultures. Our results unequivocally identify PFUR17_02294 as a lecanoric acid synthase and establish that a single lichen PKS synthesizes two phenolic rings and joins them by an ester linkage to form the depside product.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2024-11-15T03:23:24.227Z","creation":"2022-02-11T09:47:33.451Z"},"accession":"S-EPMC8365352","cross_references":{"pubmed":["34430202"],"doi":["10.1016/j.mec.2021.e00172"]}}