{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Miller RM"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["1936-1942"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8366505"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(4)"],"pubmed_abstract":["Bottom-up proteomics is currently the dominant strategy for proteome analysis. It relies critically upon the use of a protease to digest proteins into peptides, which are then identified by liquid chromatography-mass spectrometry (LC-MS). The choice of protease(s) has a substantial impact upon the utility of the bottom-up results obtained. Protease selection determines the nature of the peptides produced, which in turn affects the ability to infer the presence and quantities of the parent proteins and post-translational modifications in the sample. We present here the software tool ProteaseGuru, which provides in silico digestions by candidate proteases, allowing evaluation of their utility for bottom-up proteomic experiments. This information is useful for both studies focused on a single"],"journal":["Journal of proteome research"],"pubmed_title":["ProteaseGuru: A Tool for Protease Selection in Bottom-Up Proteomics."],"pmcid":["PMC8366505"],"funding_grant_id":["R35GM126914","R35 GM126914","T32 GM008505"],"pubmed_authors":["Ibrahim K","Miller RM","Smith LM"],"additional_accession":[]},"is_claimable":false,"name":"ProteaseGuru: A Tool for Protease Selection in Bottom-Up Proteomics.","description":"Bottom-up proteomics is currently the dominant strategy for proteome analysis. It relies critically upon the use of a protease to digest proteins into peptides, which are then identified by liquid chromatography-mass spectrometry (LC-MS). The choice of protease(s) has a substantial impact upon the utility of the bottom-up results obtained. Protease selection determines the nature of the peptides produced, which in turn affects the ability to infer the presence and quantities of the parent proteins and post-translational modifications in the sample. We present here the software tool ProteaseGuru, which provides in silico digestions by candidate proteases, allowing evaluation of their utility for bottom-up proteomic experiments. This information is useful for both studies focused on a single","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-04-08T08:44:16.284Z","creation":"2025-02-18T23:32:10.553Z"},"accession":"S-EPMC8366505","cross_references":{"pubmed":["33661641"],"doi":["10.1021/acs.jproteome.0c00954"]}}