biostudies-literatureHu WNational Key R&amp;D Program of China to Hongwen ZhouHealth Department Fund Projects of Jiangsu ProvinceNational Natural Sciences Foundation of China1316-1326https://www.ebi.ac.uk/biostudies/studies/S-EPMC8366658biostudies-literatureUnknown53(1)<h4>Objective</h4>This study aimed to investigate the effects of <i>PPM1K</i> rs1440581 and rs7678928 single nucleotide polymorphisms (SNPs) on the serum branched-chain amino acids (BCAAs) levels and cardiovascular disease (CVD) risk.<h4>Methods</h4>Anthropometric and biochemical examinations were performed at baseline and the end of 4 years in 234 individuals who were randomly recruited from the <i>Diabetes Prevention Programme in Huai'an</i> and received lifestyle intervention and follow up for 4 years. Serum BCAAs (leucine, isoleucine and valine (Val)) levels were measured by hydrophilic interaction chromatography-tandem mass spectrometric method and the <i>PPM1K</i> rs1440581 and rs7678928 were detected by high-throughput SNP genotyping at baseline. The associations of rs1440581 and rsAnnals of medicineEffects of <i>PPM1K</i> rs1440581 and rs7678928 on serum branched-chain amino acid levels and risk of cardiovascular disease.PMC8366658817007762018YFA0506904Z201317Liu ZLi JLi SHu WQi XLu YYu WWen SHao HWang XZhou HfalseEffects of <i>PPM1K</i> rs1440581 and rs7678928 on serum branched-chain amino acid levels and risk of cardiovascular disease.<h4>Objective</h4>This study aimed to investigate the effects of <i>PPM1K</i> rs1440581 and rs7678928 single nucleotide polymorphisms (SNPs) on the serum branched-chain amino acids (BCAAs) levels and cardiovascular disease (CVD) risk.<h4>Methods</h4>Anthropometric and biochemical examinations were performed at baseline and the end of 4 years in 234 individuals who were randomly recruited from the <i>Diabetes Prevention Programme in Huai'an</i> and received lifestyle intervention and follow up for 4 years. Serum BCAAs (leucine, isoleucine and valine (Val)) levels were measured by hydrophilic interaction chromatography-tandem mass spectrometric method and the <i>PPM1K</i> rs1440581 and rs7678928 were detected by high-throughput SNP genotyping at baseline. The associations of rs1440581 and rs2021-01-01T00:00:00Z2021 Dec2025-04-04T13:43:15.645Z2022-02-11T08:17:42.819ZS-EPMC83666583438249510.1080/07853890.2021.1965204